Anandamide uptake by synaptosomes from human, mouse and rat brain: Inhibition by glutamine and glutamate

Natalia Battista, Monica Bari, Alessandro Finazzi-Agrò, Mauro Maccarrone

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Anandamide (N-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal ex vivo system for the study of: i) the accumulation of AEA in brain, and ii) the pharmacological properties of AMT inhibitors. Using this ex vivo system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT.

Original languageEnglish
Article number1
Pages (from-to)1-3
Number of pages3
JournalLipids in Health and Disease
Volume1
DOIs
Publication statusPublished - Sep 3 2002

Fingerprint

Synaptosomes
Glutamine
Rats
Glutamic Acid
Brain
Membrane Transport Proteins
Lipids
Endocannabinoids
Hydrolases
anandamide
N-arachidonoylethanolamine
Pharmacology
Degradation
Enzymes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Anandamide uptake by synaptosomes from human, mouse and rat brain : Inhibition by glutamine and glutamate. / Battista, Natalia; Bari, Monica; Finazzi-Agrò, Alessandro; Maccarrone, Mauro.

In: Lipids in Health and Disease, Vol. 1, 1, 03.09.2002, p. 1-3.

Research output: Contribution to journalArticle

@article{cee8b1a0194944a4af9b69a7dad6c03d,
title = "Anandamide uptake by synaptosomes from human, mouse and rat brain: Inhibition by glutamine and glutamate",
abstract = "Anandamide (N-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called {"}endocannabinoids{"}. A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal ex vivo system for the study of: i) the accumulation of AEA in brain, and ii) the pharmacological properties of AMT inhibitors. Using this ex vivo system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT.",
author = "Natalia Battista and Monica Bari and Alessandro Finazzi-Agr{\`o} and Mauro Maccarrone",
year = "2002",
month = "9",
day = "3",
doi = "10.1186/1476-511X-1-1",
language = "English",
volume = "1",
pages = "1--3",
journal = "Lipids in Health and Disease",
issn = "1476-511X",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Anandamide uptake by synaptosomes from human, mouse and rat brain

T2 - Inhibition by glutamine and glutamate

AU - Battista, Natalia

AU - Bari, Monica

AU - Finazzi-Agrò, Alessandro

AU - Maccarrone, Mauro

PY - 2002/9/3

Y1 - 2002/9/3

N2 - Anandamide (N-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal ex vivo system for the study of: i) the accumulation of AEA in brain, and ii) the pharmacological properties of AMT inhibitors. Using this ex vivo system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT.

AB - Anandamide (N-arachidonoylethanolamine, AEA) belongs to an emerging class of endogenous lipids, called "endocannabinoids". A specific AEA membrane transporter (AMT) allows the import of this lipid and its degradation by the intracellular enzyme AEA hydrolase. Here, we show that synaptosomes from human, mouse and rat brain might be an ideal ex vivo system for the study of: i) the accumulation of AEA in brain, and ii) the pharmacological properties of AMT inhibitors. Using this ex vivo system, we demonstrate for the first time that glutamine and glutamate act as non-competitive inhibitors of AEA uptake by human, mouse and rat brain AMT.

UR - http://www.scopus.com/inward/record.url?scp=34248350364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248350364&partnerID=8YFLogxK

U2 - 10.1186/1476-511X-1-1

DO - 10.1186/1476-511X-1-1

M3 - Article

C2 - 12617751

AN - SCOPUS:34248350364

VL - 1

SP - 1

EP - 3

JO - Lipids in Health and Disease

JF - Lipids in Health and Disease

SN - 1476-511X

M1 - 1

ER -