Anaplastic lymphoma kinase (ALK) is not expressed in Hodgkin's disease: Results with ALK-11 antibody in 327 untreated patients

M. Herling, G. Z. Rassidakis, S. Viviani, V. Bonfante, R. Giardini, M. Gianni, S. W. Morris, F. Cabanillas, L. J. Medeiros, A. H. Sarris

Research output: Contribution to journalArticle

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Abstract

The t(2;5)(p23;q35) or other rare chromosomal abnormalities involving 2p23 upregulate the ALK gene, which is not expressed in normal lymphocytes. Thus, detection of ALK protein is presumptive evidence of these 2p23 abnormalities. The t(2;5) and ALK immunoreactivity are common in anaplastic large cell lymphoma of T/null-cell lineage. However, a small subset of cases of Hodgkin's disease (HD) have been reported to either carry the t(2;5) or express ALK. In this study, we have immunohistochemically evaluated 327 cases of HD with the ALK-11 antibody. ALK-11 is a well characterized polyclonal antibody raised against an intracellular portion of the ALK protein. We detected ALK-11 immunoreactivity in 8 (2.4%) cases of HD. We further studied these positive cases with ALK-1 monoclonal antibody, which reacts with an intracellular portion of ALK, similar to ALK-11. All 8 ALK-11 positive cases were negative for ALK-1. These results indicate that rare cases of HD may react with ALK-11 antibody, similar to previous reports by others using different polyclonal anti-ALK antibodies. However, the absence of ALK-1 expression in these HD cases suggests that ALK protein is not truly present and that polyclonal anti-ALK antibodies may rarely yield non-specific cross reactivity. These results further support the use of anti-ALK antibodies in the differential diagnosis of HD from ALCL.

Original languageEnglish
Pages (from-to)969-979
Number of pages11
JournalLeukemia and Lymphoma
Volume42
Issue number5
Publication statusPublished - 2001

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Hodgkin Disease
Antibodies
anaplastic lymphoma kinase
Anaplastic Large-Cell Lymphoma
Null Lymphocytes
Proteins
Cell Lineage
Chromosome Aberrations

Keywords

  • ALK
  • Hodgkin's disease
  • Immunohistochemistry ALK-11 antibody
  • t(2:5)

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Herling, M., Rassidakis, G. Z., Viviani, S., Bonfante, V., Giardini, R., Gianni, M., ... Sarris, A. H. (2001). Anaplastic lymphoma kinase (ALK) is not expressed in Hodgkin's disease: Results with ALK-11 antibody in 327 untreated patients. Leukemia and Lymphoma, 42(5), 969-979.

Anaplastic lymphoma kinase (ALK) is not expressed in Hodgkin's disease : Results with ALK-11 antibody in 327 untreated patients. / Herling, M.; Rassidakis, G. Z.; Viviani, S.; Bonfante, V.; Giardini, R.; Gianni, M.; Morris, S. W.; Cabanillas, F.; Medeiros, L. J.; Sarris, A. H.

In: Leukemia and Lymphoma, Vol. 42, No. 5, 2001, p. 969-979.

Research output: Contribution to journalArticle

Herling, M, Rassidakis, GZ, Viviani, S, Bonfante, V, Giardini, R, Gianni, M, Morris, SW, Cabanillas, F, Medeiros, LJ & Sarris, AH 2001, 'Anaplastic lymphoma kinase (ALK) is not expressed in Hodgkin's disease: Results with ALK-11 antibody in 327 untreated patients', Leukemia and Lymphoma, vol. 42, no. 5, pp. 969-979.
Herling, M. ; Rassidakis, G. Z. ; Viviani, S. ; Bonfante, V. ; Giardini, R. ; Gianni, M. ; Morris, S. W. ; Cabanillas, F. ; Medeiros, L. J. ; Sarris, A. H. / Anaplastic lymphoma kinase (ALK) is not expressed in Hodgkin's disease : Results with ALK-11 antibody in 327 untreated patients. In: Leukemia and Lymphoma. 2001 ; Vol. 42, No. 5. pp. 969-979.
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