Anatomical and cellular localization of melatonin MT1 and MT2 receptors in the adult rat brain

Baptiste Lacoste, Debora Angeloni, Sergio Dominguez-Lopez, Sara Calderoni, Alessandro Mauro, Franco Fraschini, Laurent Descarries, Gabriella Gobbi

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The involvement of melatonin in mammalian brain pathophysiology has received growing interest, but information about the anatomical distribution of its two G-protein-coupled receptors, MT1 and MT2, remains elusive. In this study, using specific antibodies, we examined the precise distribution of both melatonin receptors immunoreactivity across the adult rat brain using light, confocal, and electron microscopy. Our results demonstrate a selective MT1 and MT2 localization on neuronal cell bodies and dendrites in numerous regions of the rat telencephalon, diencephalon, and mesencephalon. Confocal and ultrastructural examination confirmed the somatodendritic nature of MT1 and MT2 receptors, both being localized on neuronal membranes. Overall, striking differences were observed in the anatomical distribution pattern of MT1 and MT2 proteins, and the labeling often appeared complementary in regions displaying both receptors. Somadendrites labeled for MT1 were observed for instance in the retrosplenial cortex, the dentate gyrus of the hippocampus, the islands of Calleja, the medial habenula, the suprachiasmatic nucleus, the superior colliculus, the substantia nigra pars compacta, the dorsal raphe nucleus, and the pars tuberalis of the pituitary gland. Somadendrites endowed with MT2 receptors were mostly observed in the CA3 field of the hippocampus, the reticular thalamic nucleus, the supraoptic nucleus, the inferior colliculus, the substantia nigra pars reticulata, and the ventrolateral periaqueductal gray. Together, these data provide the first detailed neurocytological mapping of melatonin receptors in the adult rat brain, an essential prerequisite for a better understanding of melatonin distinct receptor function and neurophysiology.

Original languageEnglish
Pages (from-to)397-417
Number of pages21
JournalJournal of Pineal Research
Volume58
Issue number4
DOIs
Publication statusPublished - May 1 2015

Fingerprint

Melatonin MT1 Receptor
Melatonin MT2 Receptor
Melatonin Receptors
Islands of Calleja
Brain
Hippocampal CA3 Region
Habenula
Inferior Colliculi
Supraoptic Nucleus
Thalamic Nuclei
Diencephalon
Telencephalon
Parahippocampal Gyrus
Periaqueductal Gray
Neurophysiology
Suprachiasmatic Nucleus
Superior Colliculi
Dentate Gyrus
Pituitary Gland
Melatonin

Keywords

  • brain
  • immunohistochemistry
  • melatonin receptors
  • neuroanatomy
  • rat

ASJC Scopus subject areas

  • Endocrinology
  • Medicine(all)

Cite this

Anatomical and cellular localization of melatonin MT1 and MT2 receptors in the adult rat brain. / Lacoste, Baptiste; Angeloni, Debora; Dominguez-Lopez, Sergio; Calderoni, Sara; Mauro, Alessandro; Fraschini, Franco; Descarries, Laurent; Gobbi, Gabriella.

In: Journal of Pineal Research, Vol. 58, No. 4, 01.05.2015, p. 397-417.

Research output: Contribution to journalArticle

Lacoste, B, Angeloni, D, Dominguez-Lopez, S, Calderoni, S, Mauro, A, Fraschini, F, Descarries, L & Gobbi, G 2015, 'Anatomical and cellular localization of melatonin MT1 and MT2 receptors in the adult rat brain', Journal of Pineal Research, vol. 58, no. 4, pp. 397-417. https://doi.org/10.1111/jpi.12224
Lacoste, Baptiste ; Angeloni, Debora ; Dominguez-Lopez, Sergio ; Calderoni, Sara ; Mauro, Alessandro ; Fraschini, Franco ; Descarries, Laurent ; Gobbi, Gabriella. / Anatomical and cellular localization of melatonin MT1 and MT2 receptors in the adult rat brain. In: Journal of Pineal Research. 2015 ; Vol. 58, No. 4. pp. 397-417.
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