Anatomical investigation of potential contacts between climbing fibers and cerebellar golgi cells in the mouse

Elisa Galliano, Marco Baratella, Martina Sgritta, Tom J H Ruigrok, Elize D. Haasdijk, Freek E. Hoebeek, Egidio D'Angelo, Dick Jaarsma, Chris I. De Zeeuw

Research output: Contribution to journalArticle

Abstract

Climbing fibers (CFs) originating in the inferior olive (IO) constitute one of the main inputs to the cerebellum. In the mammalian cerebellar cortex each of them climbs into the dendritic tree of up to ten Purkinje cells where they make hundreds of synaptic contacts and elicit the socalled all-or-none complex spikes controlling the output. While it has been proven that CFs contact molecular layer interneurons (MLIs) via spillover mechanisms, it remains to be elucidated to what extent CFs contact the main type of interneuron in the granular layer, i.e. the Golgi cells (GoCs). This issue is particularly relevant, because direct contacts would imply that CFs can also control computations at the input stage of the cerebellar cortical network. Here, we performed a systematic morphological investigation of labeled CFs and GoCs at the light microscopic level following their path and localization through the neuropil in both the granular and molecular layer. Whereas the appositions of CFs to Purkinje cells, stellate cells and basket cells in the molecular layer were prominent and numerous, those to cell-bodies and dendrites of GoCs in both the granular layer and molecular layer were virtually absent. Our results argue against the functional significance of direct synaptic contacts between CFs and interneurons at the input stage, but support those at the output stage.

Original languageEnglish
JournalFrontiers in Neural Circuits
Issue numberMAR
DOIs
Publication statusPublished - Mar 14 2013

Keywords

  • Cerebellum
  • Climbing fiber
  • Confocal microscopy
  • Golgi cells
  • Synapse

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Cellular and Molecular Neuroscience
  • Sensory Systems
  • Cognitive Neuroscience

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    Galliano, E., Baratella, M., Sgritta, M., Ruigrok, T. J. H., Haasdijk, E. D., Hoebeek, F. E., D'Angelo, E., Jaarsma, D., & De Zeeuw, C. I. (2013). Anatomical investigation of potential contacts between climbing fibers and cerebellar golgi cells in the mouse. Frontiers in Neural Circuits, (MAR). https://doi.org/10.3389/fncir.2013.00059