Anchorage dependence of mitogen-induced G1 to S transition in primary T lymphocytes

J. Geginat, G. Bossi, J. R. Bender, R. Pardi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Anchorage dependence defines the cellular requirement for integrin- mediated adhesion to substrate to initiate DNA replication in response to growth factors. In this study we investigated whether normal T cells, which spend extended periods in a nonadherent state, show similar requirements for cell cycle progression in response to TCR stimulation. Resting primary T lymphocytes were induced to enter the cell cycle by TCR triggering, and leukocyte integrins were either engaged using purified ICAM-1 or inhibited with function-blocking mAbs. Our data indicate that leukocyte integrins complement TCR-driven mitogenic signals not as a result of their direct clustering but, rather, via integrin-dependent organization of the actin cytoskeleton. Leukocyte integrin-dependent reorganization of the actin cytoskeleton cooperates with the TCR to effect mitogen-activated protein kinase activation, but also represents a required late (4-8 h poststimulation) component in the mitogenic response of normal T cells. Prolonged leukocyte integrin-dependent spreading, in the context of intercellular contact, is a requisite for the production of the mitogenic cytokine IL-2, which, in turn, is involved in the induction of D3 cyclin and is primarily responsible for the decrease in the cyclin-dependent kinase inhibitor p27(kip), resulting in retinoblastoma protein inactivation and S phase entry. Thus, T lymphocytes represent a peculiar case of anchorage dependence, in which signals conveyed by integrins act sequentially with the activating stimulus to effect a sustained production of the essential mitogenic cytokine.

Original languageEnglish
Pages (from-to)5085-5093
Number of pages9
JournalJournal of Immunology
Volume162
Issue number9
Publication statusPublished - May 1 1999

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Mitogens
Integrins
T-Lymphocytes
Leukocytes
Actin Cytoskeleton
Cell Cycle
Cyclin D3
Cyclin-Dependent Kinase Inhibitor p27
Cytokines
Retinoblastoma Protein
Protein S
Intercellular Adhesion Molecule-1
Mitogen-Activated Protein Kinases
DNA Replication
S Phase
Interleukin-2
Cluster Analysis
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Anchorage dependence of mitogen-induced G1 to S transition in primary T lymphocytes. / Geginat, J.; Bossi, G.; Bender, J. R.; Pardi, R.

In: Journal of Immunology, Vol. 162, No. 9, 01.05.1999, p. 5085-5093.

Research output: Contribution to journalArticle

Geginat, J. ; Bossi, G. ; Bender, J. R. ; Pardi, R. / Anchorage dependence of mitogen-induced G1 to S transition in primary T lymphocytes. In: Journal of Immunology. 1999 ; Vol. 162, No. 9. pp. 5085-5093.
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