Androgen- and insulin-related gene signature using a specific low density oligoarray "androchip 2" in peripheral blood mononuclear cells in agonists, recreational athletes and sedentary subjects

D. Minella, M. Biancolella, B. Testa, G. Prosperini, R. Zenobi, Giuseppe Novelli, M. G. Giganti

Research output: Contribution to journalArticle

Abstract

The early detection of genomic biomarkers (e.g. RNAs) through analysis of circulating blood cells could have a substantial impact on biomedicine, particularly in monitoring clinical trials, drug toxicity and doping in athletes. To achieve this goal, it is essential to develop methods that are sufficiently sensitive to detect biomarker alterations during normal biologic processes, pathogenic processes, and/or in response to therapeutic or other intervention. Using a low density microarray ("AndroChip 2") we detected a "transcriptional profiling signature" of 190 genes related to androgen and insulin metabolism pathway, in peripheral blood mononuclear cells (PBMC) in subjects with different intensities of sports activities. We demonstrated that androgen and insulin gene transcriptional levels are independent to sports activity and therefore potentially suitable for drug monitoring and/or drug doping (such as anabolic androgen steroid AAS abuse) and/or gene doping.

Original languageEnglish
Pages (from-to)413-423
Number of pages11
JournalJournal of Biological Regulators and Homeostatic Agents
Volume24
Issue number4
Publication statusPublished - Oct 2010

Fingerprint

Athletes
Androgens
Blood Cells
Insulin
Sports
Testosterone Congeners
Biomarkers
Genes
Drug Monitoring
Drug-Related Side Effects and Adverse Reactions
Clinical Trials
RNA
Pharmaceutical Preparations
Therapeutics

Keywords

  • Anabolic androgen steroid
  • Microarray
  • Peripheral blood mononuclear cells

ASJC Scopus subject areas

  • Oncology
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)
  • Immunology and Allergy
  • Immunology
  • Endocrinology
  • Physiology
  • Cancer Research

Cite this

Androgen- and insulin-related gene signature using a specific low density oligoarray "androchip 2" in peripheral blood mononuclear cells in agonists, recreational athletes and sedentary subjects. / Minella, D.; Biancolella, M.; Testa, B.; Prosperini, G.; Zenobi, R.; Novelli, Giuseppe; Giganti, M. G.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 24, No. 4, 10.2010, p. 413-423.

Research output: Contribution to journalArticle

@article{64fdd7ee143c4dc390ce27b456114f36,
title = "Androgen- and insulin-related gene signature using a specific low density oligoarray {"}androchip 2{"} in peripheral blood mononuclear cells in agonists, recreational athletes and sedentary subjects",
abstract = "The early detection of genomic biomarkers (e.g. RNAs) through analysis of circulating blood cells could have a substantial impact on biomedicine, particularly in monitoring clinical trials, drug toxicity and doping in athletes. To achieve this goal, it is essential to develop methods that are sufficiently sensitive to detect biomarker alterations during normal biologic processes, pathogenic processes, and/or in response to therapeutic or other intervention. Using a low density microarray ({"}AndroChip 2{"}) we detected a {"}transcriptional profiling signature{"} of 190 genes related to androgen and insulin metabolism pathway, in peripheral blood mononuclear cells (PBMC) in subjects with different intensities of sports activities. We demonstrated that androgen and insulin gene transcriptional levels are independent to sports activity and therefore potentially suitable for drug monitoring and/or drug doping (such as anabolic androgen steroid AAS abuse) and/or gene doping.",
keywords = "Anabolic androgen steroid, Microarray, Peripheral blood mononuclear cells",
author = "D. Minella and M. Biancolella and B. Testa and G. Prosperini and R. Zenobi and Giuseppe Novelli and Giganti, {M. G.}",
year = "2010",
month = "10",
language = "English",
volume = "24",
pages = "413--423",
journal = "Journal of Biological Regulators and Homeostatic Agents",
issn = "0393-974X",
publisher = "Biolife s.a.s.",
number = "4",

}

TY - JOUR

T1 - Androgen- and insulin-related gene signature using a specific low density oligoarray "androchip 2" in peripheral blood mononuclear cells in agonists, recreational athletes and sedentary subjects

AU - Minella, D.

AU - Biancolella, M.

AU - Testa, B.

AU - Prosperini, G.

AU - Zenobi, R.

AU - Novelli, Giuseppe

AU - Giganti, M. G.

PY - 2010/10

Y1 - 2010/10

N2 - The early detection of genomic biomarkers (e.g. RNAs) through analysis of circulating blood cells could have a substantial impact on biomedicine, particularly in monitoring clinical trials, drug toxicity and doping in athletes. To achieve this goal, it is essential to develop methods that are sufficiently sensitive to detect biomarker alterations during normal biologic processes, pathogenic processes, and/or in response to therapeutic or other intervention. Using a low density microarray ("AndroChip 2") we detected a "transcriptional profiling signature" of 190 genes related to androgen and insulin metabolism pathway, in peripheral blood mononuclear cells (PBMC) in subjects with different intensities of sports activities. We demonstrated that androgen and insulin gene transcriptional levels are independent to sports activity and therefore potentially suitable for drug monitoring and/or drug doping (such as anabolic androgen steroid AAS abuse) and/or gene doping.

AB - The early detection of genomic biomarkers (e.g. RNAs) through analysis of circulating blood cells could have a substantial impact on biomedicine, particularly in monitoring clinical trials, drug toxicity and doping in athletes. To achieve this goal, it is essential to develop methods that are sufficiently sensitive to detect biomarker alterations during normal biologic processes, pathogenic processes, and/or in response to therapeutic or other intervention. Using a low density microarray ("AndroChip 2") we detected a "transcriptional profiling signature" of 190 genes related to androgen and insulin metabolism pathway, in peripheral blood mononuclear cells (PBMC) in subjects with different intensities of sports activities. We demonstrated that androgen and insulin gene transcriptional levels are independent to sports activity and therefore potentially suitable for drug monitoring and/or drug doping (such as anabolic androgen steroid AAS abuse) and/or gene doping.

KW - Anabolic androgen steroid

KW - Microarray

KW - Peripheral blood mononuclear cells

UR - http://www.scopus.com/inward/record.url?scp=79952278172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952278172&partnerID=8YFLogxK

M3 - Article

C2 - 21122280

AN - SCOPUS:79952278172

VL - 24

SP - 413

EP - 423

JO - Journal of Biological Regulators and Homeostatic Agents

JF - Journal of Biological Regulators and Homeostatic Agents

SN - 0393-974X

IS - 4

ER -