Androgen- and insulin-related gene signature using a specific low density oligoarray "androchip 2" in peripheral blood mononuclear cells in agonists, recreational athletes and sedentary subjects

D. Minella, M. Biancolella, B. Testa, G. Prosperini, R. Zenobi, Giuseppe Novelli, M. G. Giganti

Research output: Contribution to journalArticlepeer-review

Abstract

The early detection of genomic biomarkers (e.g. RNAs) through analysis of circulating blood cells could have a substantial impact on biomedicine, particularly in monitoring clinical trials, drug toxicity and doping in athletes. To achieve this goal, it is essential to develop methods that are sufficiently sensitive to detect biomarker alterations during normal biologic processes, pathogenic processes, and/or in response to therapeutic or other intervention. Using a low density microarray ("AndroChip 2") we detected a "transcriptional profiling signature" of 190 genes related to androgen and insulin metabolism pathway, in peripheral blood mononuclear cells (PBMC) in subjects with different intensities of sports activities. We demonstrated that androgen and insulin gene transcriptional levels are independent to sports activity and therefore potentially suitable for drug monitoring and/or drug doping (such as anabolic androgen steroid AAS abuse) and/or gene doping.

Original languageEnglish
Pages (from-to)413-423
Number of pages11
JournalJournal of Biological Regulators and Homeostatic Agents
Volume24
Issue number4
Publication statusPublished - Oct 2010

Keywords

  • Anabolic androgen steroid
  • Microarray
  • Peripheral blood mononuclear cells

ASJC Scopus subject areas

  • Oncology
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)
  • Immunology and Allergy
  • Immunology
  • Endocrinology
  • Physiology
  • Cancer Research

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