Abstract
Anabolic androgenic steroids, a class of steroid hormones related to testosterone, are natural ligands of androgen receptor (AR), a member of the nuclear receptor superfamily of ligand-activated transcription factors. AR binds specific DNA elements, known as androgen-response elements. Testosterone, the main male sexual hormone, binds AR directly and indirectly, through conversion into dihydrotestosterone (DHT), its more active metabolite. Anabolic androgenic steroids are frequently detected in the urine of doped athletes; their consumption is also growing among sport amateurs and adolescents. The effects of androgens can differ depending on the target cells and/or tissues. To gain insight into transcription activation mechanisms of AR, we investigated AR protein signaling in human peripheral blood lymphocytes treated with supraphysiological doses of DHT. We performed a comparative proteomic analysis and we identified about 30 differentially expressed proteins. At least five species contained a consensus androgen-response elements sequence in the promoter region of related coding genes. The analysis also revealed that high doses of DHT activate the drug detoxification process, could stimulate an increase in cell motility and exert a prosurvival effect rather than an apoptotic one.
| Original language | English |
|---|---|
| Pages (from-to) | 3165-3175 |
| Number of pages | 11 |
| Journal | Proteomics |
| Volume | 10 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - Sep 2010 |
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Keywords
- 2-D DIGE
- Androgen receptor
- Cell biology
- Dihydrotestosterone
- Lymphocytes
- Steroids abuse
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry
Cite this
Androgen receptor signaling induced by supraphysiological doses of dihydrotestosterone in human peripheral blood lymphocytes. / Imperlini, Esther; Mancini, Annamaria; Spaziani, Sara; Martone, Domenico; Alfieri, Andreina; Gemei, Marica; Del Vecchio, Luigi; Buono, Pasqualina; Orrù, Stefania.
In: Proteomics, Vol. 10, No. 17, 09.2010, p. 3165-3175.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Androgen receptor signaling induced by supraphysiological doses of dihydrotestosterone in human peripheral blood lymphocytes
AU - Imperlini, Esther
AU - Mancini, Annamaria
AU - Spaziani, Sara
AU - Martone, Domenico
AU - Alfieri, Andreina
AU - Gemei, Marica
AU - Del Vecchio, Luigi
AU - Buono, Pasqualina
AU - Orrù, Stefania
PY - 2010/9
Y1 - 2010/9
N2 - Anabolic androgenic steroids, a class of steroid hormones related to testosterone, are natural ligands of androgen receptor (AR), a member of the nuclear receptor superfamily of ligand-activated transcription factors. AR binds specific DNA elements, known as androgen-response elements. Testosterone, the main male sexual hormone, binds AR directly and indirectly, through conversion into dihydrotestosterone (DHT), its more active metabolite. Anabolic androgenic steroids are frequently detected in the urine of doped athletes; their consumption is also growing among sport amateurs and adolescents. The effects of androgens can differ depending on the target cells and/or tissues. To gain insight into transcription activation mechanisms of AR, we investigated AR protein signaling in human peripheral blood lymphocytes treated with supraphysiological doses of DHT. We performed a comparative proteomic analysis and we identified about 30 differentially expressed proteins. At least five species contained a consensus androgen-response elements sequence in the promoter region of related coding genes. The analysis also revealed that high doses of DHT activate the drug detoxification process, could stimulate an increase in cell motility and exert a prosurvival effect rather than an apoptotic one.
AB - Anabolic androgenic steroids, a class of steroid hormones related to testosterone, are natural ligands of androgen receptor (AR), a member of the nuclear receptor superfamily of ligand-activated transcription factors. AR binds specific DNA elements, known as androgen-response elements. Testosterone, the main male sexual hormone, binds AR directly and indirectly, through conversion into dihydrotestosterone (DHT), its more active metabolite. Anabolic androgenic steroids are frequently detected in the urine of doped athletes; their consumption is also growing among sport amateurs and adolescents. The effects of androgens can differ depending on the target cells and/or tissues. To gain insight into transcription activation mechanisms of AR, we investigated AR protein signaling in human peripheral blood lymphocytes treated with supraphysiological doses of DHT. We performed a comparative proteomic analysis and we identified about 30 differentially expressed proteins. At least five species contained a consensus androgen-response elements sequence in the promoter region of related coding genes. The analysis also revealed that high doses of DHT activate the drug detoxification process, could stimulate an increase in cell motility and exert a prosurvival effect rather than an apoptotic one.
KW - 2-D DIGE
KW - Androgen receptor
KW - Cell biology
KW - Dihydrotestosterone
KW - Lymphocytes
KW - Steroids abuse
UR - http://www.scopus.com/inward/record.url?scp=77956125387&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956125387&partnerID=8YFLogxK
U2 - 10.1002/pmic.201000079
DO - 10.1002/pmic.201000079
M3 - Article
C2 - 20677326
AN - SCOPUS:77956125387
VL - 10
SP - 3165
EP - 3175
JO - Proteomics
JF - Proteomics
SN - 1615-9853
IS - 17
ER -