Abstract
Androgen recptors are expressed at a low level in the cell line PC-3, which does not respond to either androgens or antiandrogens. If these cells are exposed to natural beta-interferon (β-IFN) a reduction in cell growth and an increase in androgen receptors, evaluated by both biochemical and immunocytochemical techniques, occur. This increase seems not to be related to a selective block of PC-3 in any phase of the cell cycle. Pretreatment with β-IFN determines in PC-3 cells a partial responsiveness to the androgen dihydrotestosterone as reflected by the increase in cell number. Moreover, the antiandrogen hydroxyflutamide shows agonistic properties by increasing the cell number of PC-3 cells pre-exposed to β-IFN. When the antiandrogen is tested in combination with interferon, it produces a reduction in the β-IFN-induced inhibition of cell growth. It is not known whether these unexpected effects are due to the increase in androgen receptors or to other mechanisms.
| Original language | English |
|---|---|
| Pages (from-to) | 33-38 |
| Number of pages | 6 |
| Journal | Urological Research |
| Volume | 22 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1991 |
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Keywords
- Androgen receptors
- Hormone sensitivity
- Hydroxyflutamide
- Interferon
- Prostatic cancer
ASJC Scopus subject areas
- Urology
Cite this
Androgen receptors and hormone sensitivity of a human prostatic cancer cell line (PC-3) are modulated by natural beta-interferon. / Sica, G.; Dell'Acqua, G.; Iacopino, F.; Fattorossi, A.; Marchetti, P.; van der Kwast, T. H.; Pavone-Macaluso, M.
In: Urological Research, Vol. 22, No. 1, 1991, p. 33-38.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Androgen receptors and hormone sensitivity of a human prostatic cancer cell line (PC-3) are modulated by natural beta-interferon
AU - Sica, G.
AU - Dell'Acqua, G.
AU - Iacopino, F.
AU - Fattorossi, A.
AU - Marchetti, P.
AU - van der Kwast, T. H.
AU - Pavone-Macaluso, M.
PY - 1991
Y1 - 1991
N2 - Androgen recptors are expressed at a low level in the cell line PC-3, which does not respond to either androgens or antiandrogens. If these cells are exposed to natural beta-interferon (β-IFN) a reduction in cell growth and an increase in androgen receptors, evaluated by both biochemical and immunocytochemical techniques, occur. This increase seems not to be related to a selective block of PC-3 in any phase of the cell cycle. Pretreatment with β-IFN determines in PC-3 cells a partial responsiveness to the androgen dihydrotestosterone as reflected by the increase in cell number. Moreover, the antiandrogen hydroxyflutamide shows agonistic properties by increasing the cell number of PC-3 cells pre-exposed to β-IFN. When the antiandrogen is tested in combination with interferon, it produces a reduction in the β-IFN-induced inhibition of cell growth. It is not known whether these unexpected effects are due to the increase in androgen receptors or to other mechanisms.
AB - Androgen recptors are expressed at a low level in the cell line PC-3, which does not respond to either androgens or antiandrogens. If these cells are exposed to natural beta-interferon (β-IFN) a reduction in cell growth and an increase in androgen receptors, evaluated by both biochemical and immunocytochemical techniques, occur. This increase seems not to be related to a selective block of PC-3 in any phase of the cell cycle. Pretreatment with β-IFN determines in PC-3 cells a partial responsiveness to the androgen dihydrotestosterone as reflected by the increase in cell number. Moreover, the antiandrogen hydroxyflutamide shows agonistic properties by increasing the cell number of PC-3 cells pre-exposed to β-IFN. When the antiandrogen is tested in combination with interferon, it produces a reduction in the β-IFN-induced inhibition of cell growth. It is not known whether these unexpected effects are due to the increase in androgen receptors or to other mechanisms.
KW - Androgen receptors
KW - Hormone sensitivity
KW - Hydroxyflutamide
KW - Interferon
KW - Prostatic cancer
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UR - http://www.scopus.com/inward/citedby.url?scp=0028217260&partnerID=8YFLogxK
U2 - 10.1007/BF00431546
DO - 10.1007/BF00431546
M3 - Article
C2 - 8073539
AN - SCOPUS:0028217260
VL - 22
SP - 33
EP - 38
JO - Urological Research
JF - Urological Research
SN - 0300-5623
IS - 1
ER -