Angiogenesis correlates with macrophage and mast cell infiltration in lung tissue of animals exposed to fluoro-edenite fibers

Giuseppe Musumeci, Carla Loreto, Salvatore Giunta, Venerando Rapisarda, Marta Anna Szychlinska, Rosa Imbesi, Alessandro Castorina, Tiziana Annese, Sergio Castorina, Paola Castrogiovanni, Domenico Ribatti

Research output: Contribution to journalArticle

Abstract

Angiogenesis plays a crucial role in progression of pleural malignant mesothelioma. A significantly increased incidence of pleural mesothelioma has been attributed to exposure to fluoro-edenite, a fibrous amphibole extracted from a local stone quarry. In this study, we have investigated the expression of CD68-positive macrophages, tryptase-positive mast cells and CD31 positive areas, as expression of microvascular density, in lung tissue of sheeps exposed to fluoro-edenite fibers vs controls, by immunohistochemical, morphometric and Western blot analysis. The result have evidenced a significant increase in the expression of CD68-positive macrophages, tryptase-positive mast cells as well as a significant increase in microvascular density evaluated as CD31 positive areas in lung tissue of of sheeps exposed to fluoro-edenite fibers vs controls. These data confirmed the important role played by tumor microenvironment components, including macrophages and mast cells, in favour of angiogenesis in pleural mesothelioma induced by fluoro-edenite exposure.

Original languageEnglish
Pages (from-to)91-98
JournalExperimental Cell Research
Volume346
Issue number1
DOIs
Publication statusAccepted/In press - Mar 21 2016

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Keywords

  • Angiogenesis
  • Fluoro-edenite
  • Macrophages
  • Mast cells
  • Mesothelioma

ASJC Scopus subject areas

  • Cell Biology

Cite this

Musumeci, G., Loreto, C., Giunta, S., Rapisarda, V., Szychlinska, M. A., Imbesi, R., Castorina, A., Annese, T., Castorina, S., Castrogiovanni, P., & Ribatti, D. (Accepted/In press). Angiogenesis correlates with macrophage and mast cell infiltration in lung tissue of animals exposed to fluoro-edenite fibers. Experimental Cell Research, 346(1), 91-98. https://doi.org/10.1016/j.yexcr.2016.06.017