Abstract
We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.
| Original language | English |
|---|---|
| Pages (from-to) | 1023-1028 |
| Number of pages | 6 |
| Journal | Journal of Cellular and Molecular Medicine |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 2008 |
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Keywords
- Angiogenesis
- Chorioallantoic membrane
- Endostatin
- Multiple myeloma
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin. / Mangieri, Domenica; Nico, Beatrice; Benagiano, Vincenzo; De Giorgis, Michela; Vacca, Angelo; Ribatti, Domenico.
In: Journal of Cellular and Molecular Medicine, Vol. 12, No. 3, 06.2008, p. 1023-1028.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin
AU - Mangieri, Domenica
AU - Nico, Beatrice
AU - Benagiano, Vincenzo
AU - De Giorgis, Michela
AU - Vacca, Angelo
AU - Ribatti, Domenico
PY - 2008/6
Y1 - 2008/6
N2 - We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.
AB - We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.
KW - Angiogenesis
KW - Chorioallantoic membrane
KW - Endostatin
KW - Multiple myeloma
UR - http://www.scopus.com/inward/record.url?scp=44149087862&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44149087862&partnerID=8YFLogxK
U2 - 10.1111/j.1582-4934.2008.00152.x
DO - 10.1111/j.1582-4934.2008.00152.x
M3 - Article
C2 - 18494942
AN - SCOPUS:44149087862
VL - 12
SP - 1023
EP - 1028
JO - Journal of Cellular and Molecular Medicine
JF - Journal of Cellular and Molecular Medicine
SN - 1582-1838
IS - 3
ER -