Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin

Domenica Mangieri; Beatrice Nico; Vincenzo Benagiano; Michela De Giorgis; Angelo Vacca; Domenico Ribatti

doi:10.1111/j.1582-4934.2008.00152.x

Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin

Domenica Mangieri, Beatrice Nico, Vincenzo Benagiano, Michela De Giorgis, Angelo Vacca, Domenico Ribatti

IRCCS Giovanni Paolo II

Research output: Contribution to journal › Article › peer-review

Abstract

We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

Original language	English
Pages (from-to)	1023-1028
Number of pages	6
Journal	Journal of Cellular and Molecular Medicine
Volume	12
Issue number	3
DOIs	https://doi.org/10.1111/j.1582-4934.2008.00152.x
Publication status	Published - Jun 2008

Keywords

Angiogenesis
Chorioallantoic membrane
Endostatin
Multiple myeloma

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Biochemistry

Access to Document

10.1111/j.1582-4934.2008.00152.x

Cite this

Mangieri, D., Nico, B., Benagiano, V., De Giorgis, M., Vacca, A., & Ribatti, D. (2008). Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin. Journal of Cellular and Molecular Medicine, 12(3), 1023-1028. https://doi.org/10.1111/j.1582-4934.2008.00152.x

Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin. / Mangieri, Domenica; Nico, Beatrice; Benagiano, Vincenzo; De Giorgis, Michela; Vacca, Angelo; Ribatti, Domenico.

In: Journal of Cellular and Molecular Medicine, Vol. 12, No. 3, 06.2008, p. 1023-1028.

Research output: Contribution to journal › Article › peer-review

Mangieri, D, Nico, B, Benagiano, V, De Giorgis, M, Vacca, A & Ribatti, D 2008, 'Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin', Journal of Cellular and Molecular Medicine, vol. 12, no. 3, pp. 1023-1028. https://doi.org/10.1111/j.1582-4934.2008.00152.x

Mangieri D, Nico B, Benagiano V, De Giorgis M, Vacca A, Ribatti D. Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin. Journal of Cellular and Molecular Medicine. 2008 Jun;12(3):1023-1028. https://doi.org/10.1111/j.1582-4934.2008.00152.x

Mangieri, Domenica ; Nico, Beatrice ; Benagiano, Vincenzo ; De Giorgis, Michela ; Vacca, Angelo ; Ribatti, Domenico. / Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin. In: Journal of Cellular and Molecular Medicine. 2008 ; Vol. 12, No. 3. pp. 1023-1028.

@article{2709b73e1643426b8431beedc622f3e6,

title = "Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin",

abstract = "We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.",

keywords = "Angiogenesis, Chorioallantoic membrane, Endostatin, Multiple myeloma",

author = "Domenica Mangieri and Beatrice Nico and Vincenzo Benagiano and {De Giorgis}, Michela and Angelo Vacca and Domenico Ribatti",

year = "2008",

month = jun,

doi = "10.1111/j.1582-4934.2008.00152.x",

language = "English",

volume = "12",

pages = "1023--1028",

journal = "Journal of Cellular and Molecular Medicine",

issn = "1582-1838",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin

AU - Mangieri, Domenica

AU - Nico, Beatrice

AU - Benagiano, Vincenzo

AU - De Giorgis, Michela

AU - Vacca, Angelo

AU - Ribatti, Domenico

PY - 2008/6

Y1 - 2008/6

N2 - We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

AB - We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

KW - Angiogenesis

KW - Chorioallantoic membrane

KW - Endostatin

KW - Multiple myeloma

UR - http://www.scopus.com/inward/record.url?scp=44149087862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44149087862&partnerID=8YFLogxK

U2 - 10.1111/j.1582-4934.2008.00152.x

DO - 10.1111/j.1582-4934.2008.00152.x

M3 - Article

C2 - 18494942

AN - SCOPUS:44149087862

VL - 12

SP - 1023

EP - 1028

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

SN - 1582-1838

IS - 3

ER -

Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this