Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin

Domenica Mangieri, Beatrice Nico, Vincenzo Benagiano, Michela De Giorgis, Angelo Vacca, Domenico Ribatti

Research output: Contribution to journalArticle

Abstract

We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

Original languageEnglish
Pages (from-to)1023-1028
Number of pages6
JournalJournal of Cellular and Molecular Medicine
Volume12
Issue number3
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Angiogenesis
  • Chorioallantoic membrane
  • Endostatin
  • Multiple myeloma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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