TY - JOUR
T1 - Angiogenic and anti-inflammatory properties of mesenchymal stem cells from cord blood
T2 - Soluble factors and extracellular vesicles for cell regeneration
AU - Montemurro, Tiziana
AU - Viganò, Mariele
AU - Ragni, Enrico
AU - Barilani, Mario
AU - Parazzi, Valentina
AU - Boldrin, Valentina
AU - Lavazza, Cristiana
AU - Montelatici, Elisa
AU - Banfi, Federica
AU - Lauri, Eleonora
AU - Giovanelli, Silvia
AU - Baccarin, Marco
AU - Guerneri, Silvana
AU - Giordano, Rosaria
AU - Lazzari, Lorenza
PY - 2016
Y1 - 2016
N2 - In a recent work, our group showed the existence of two distinct mesenchymal stem cell (MSC) subsets within human umbilical cord blood. One less proliferative and short-living (SL-CBMSC), the other with higher growth rate and long-living (LL-CBMSC), and therefore better suited for regenerative medicine applications. We examined whether LL-CBMSC possess peculiar paracrine properties able to affect angiogenesis or inflammatory processes. It was shown for the first time that pro-angiogenic, proliferation-stimulating and tissue repairing factors were released at high level not only as soluble cytokines, but also as mRNA precursors embedded in membrane vesicles. The combination of this primary (proteic factors interacting with surface receptors) and delayed (mRNA transferred and translated . via vesicle fusion and cargo release) interaction in endothelial target cells resulted in strong blood vessel induction with the development of capillary-like structures. In addition, LL-CBMSC dynamically modulated their release of pro-angiogenic and anti-inflammatory factors in an . in vitro model of damage. In conclusion, LL-CBMSC synthesize and secrete multiple factors that may be attuned in response to the status of the target cell, a crucial requisite when paracrine mechanisms are needed at onset of tissue regeneration.
AB - In a recent work, our group showed the existence of two distinct mesenchymal stem cell (MSC) subsets within human umbilical cord blood. One less proliferative and short-living (SL-CBMSC), the other with higher growth rate and long-living (LL-CBMSC), and therefore better suited for regenerative medicine applications. We examined whether LL-CBMSC possess peculiar paracrine properties able to affect angiogenesis or inflammatory processes. It was shown for the first time that pro-angiogenic, proliferation-stimulating and tissue repairing factors were released at high level not only as soluble cytokines, but also as mRNA precursors embedded in membrane vesicles. The combination of this primary (proteic factors interacting with surface receptors) and delayed (mRNA transferred and translated . via vesicle fusion and cargo release) interaction in endothelial target cells resulted in strong blood vessel induction with the development of capillary-like structures. In addition, LL-CBMSC dynamically modulated their release of pro-angiogenic and anti-inflammatory factors in an . in vitro model of damage. In conclusion, LL-CBMSC synthesize and secrete multiple factors that may be attuned in response to the status of the target cell, a crucial requisite when paracrine mechanisms are needed at onset of tissue regeneration.
KW - Angiogenesis
KW - Cord blood
KW - Extracellular vesicles
KW - Growth factors
KW - Human mesenchymal stem cells
KW - MiRNA
UR - http://www.scopus.com/inward/record.url?scp=84964664293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84964664293&partnerID=8YFLogxK
U2 - 10.1016/j.ejcb.2016.04.003
DO - 10.1016/j.ejcb.2016.04.003
M3 - Article
AN - SCOPUS:84964664293
SP - 228
EP - 238
JO - European Journal of Cell Biology
JF - European Journal of Cell Biology
SN - 0171-9335
ER -