TY - JOUR
T1 - Angiogenic cells, macroparticles and RNA transcripts in laparoscopic vs. open surgery for colorectal cancer
AU - Bono, Anna
AU - Bianchi, Paolo P.
AU - Locatelli, Andrea
AU - Calleri, Angelica
AU - Quarna, Jessica
AU - Antoniotti, Pier L.
AU - Rabascio, Cristina
AU - Mancuso, Patrizia
AU - Andreoni, Bruno
AU - Bertolini, Francesco
PY - 2010/10/1
Y1 - 2010/10/1
N2 - Background: Angiogenesis is crucial for tissue repair and cancer progression. We investigated a panel of angiogenic cells, macroparticles and RNA transcripts before, during and after laparoscopic colectomy or open colectomy for colorectal cancer. Results: Viable and apoptotic circulating endothelial cells were significantly increased after open but not after laparoscopic colectomy (p <0.01). A significant decrease of circulating mRNA coding for VEGFR-C and D and PDGFR-β was found after laparoscopic but not after open colectomy. Methods: A total of 24 patients were enrolled. Viable and apoptotic circulating endothelial cells, progenitors and macroparticles were evaluated by flow cytometry. The number of copies of angiogenesis-related RNA transcripts were evaluated by quantitative PCR. Conclusion: Open, but not laparoscopic colectomy, was associated with a significant post-operative increase in circulating endothelial cells, either apoptotic (likely due to surgery-related vascular damage) and viable (likely representing vascular remodeling). Circulating RNA copies coding for some angiogenic genes were significantly decreased after laparoscopic colectomy likely because of the removal of the tumor lesion. This decrease was not observed after open colectomy, were a more pronounced wave of angiogenesis related to wound healing was expected. These results indicate a relevant wave of angiogenesis-related cells and transcripts after open but not after laparoscopic colectomy.
AB - Background: Angiogenesis is crucial for tissue repair and cancer progression. We investigated a panel of angiogenic cells, macroparticles and RNA transcripts before, during and after laparoscopic colectomy or open colectomy for colorectal cancer. Results: Viable and apoptotic circulating endothelial cells were significantly increased after open but not after laparoscopic colectomy (p <0.01). A significant decrease of circulating mRNA coding for VEGFR-C and D and PDGFR-β was found after laparoscopic but not after open colectomy. Methods: A total of 24 patients were enrolled. Viable and apoptotic circulating endothelial cells, progenitors and macroparticles were evaluated by flow cytometry. The number of copies of angiogenesis-related RNA transcripts were evaluated by quantitative PCR. Conclusion: Open, but not laparoscopic colectomy, was associated with a significant post-operative increase in circulating endothelial cells, either apoptotic (likely due to surgery-related vascular damage) and viable (likely representing vascular remodeling). Circulating RNA copies coding for some angiogenic genes were significantly decreased after laparoscopic colectomy likely because of the removal of the tumor lesion. This decrease was not observed after open colectomy, were a more pronounced wave of angiogenesis related to wound healing was expected. These results indicate a relevant wave of angiogenesis-related cells and transcripts after open but not after laparoscopic colectomy.
KW - Angiogenesis
KW - Colorectal cancer
KW - Endothelial cells
KW - Growth factors
KW - Laparoscopic/open colectomy
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U2 - 10.4161/cbt.10.7.12898
DO - 10.4161/cbt.10.7.12898
M3 - Article
C2 - 20676027
AN - SCOPUS:77957585957
VL - 10
SP - 682
EP - 685
JO - Cancer Biology and Therapy
JF - Cancer Biology and Therapy
SN - 1538-4047
IS - 7
ER -