Angiotensin converting enzyme gene deletion allele is independently and strongly associated with coronary atherosclerosis and myocardial infarction

Eloisa Arbustini, Maurizia Grasso, Roberta Fasani, Catherine Klersy, Marta Diegoli, Emanuele Porcu, Nadia Banchieri, Paolo Fortina, Cesare Danesino, Giuseppe Specchia

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Abstract

Objective-To investigate the association of the three angiotensin converting enzyme (ACE) genotypes, DD, ID, and IH, with the occurrence or absence of coronary atherosclerosis and with myocardial infarction and hypertension. Design-Cohort analysis study. Setting-North-Italy reference centre. Subjects-388 white Italian patients (281 males; mean age 60-7 (SD 12-5) years) with proven coronary atherosclerosis (n = 255) or with angiographically normal coronary arteries (n = 133). A further group of 290 healthy blood donors was tested for allele frequency comparison. Interventions-ACEIID polymorphism was analysed with polymerase chain reaction on DNA from white blood cells. Main outcome measures-Coronary atherosclerosis, myocardial infarction, hypertension. Results-The D and I allele frequencies were respectively 0-63 and 0 37 in the overall healthy blood donor group and 0-66 and 0.34 in the overall study group. In the latter, univariate analysis showed (1) that coronary atherosclerosis (255 patients) was associated with the deletion allele, with an odds ratio (OR) of 5.78 for DDIII, P <0.001, and 2.39 for IDIII, P = 0-006; and (2) that myocardial infarction (154 patients) was associated with the DD genotype (OR DDIII = 2-56, P = 0.007), but not with the ID genotype (OR DDIII = 196, P = 0.056). Finally, hypertension proved to be unrelated with the ACE genotype. The distribution between the three genotypes of known risk factors for coronary artery disease was similar. Logistic regression modelling, performed to test the association of the selected risk factors simultaneously with coronary atherosclerosis and myocardial infarction, showed that the deletion allele (whether DD or ID) was the strongest risk factor for atherosclerosis, and that the D allele was significantly associated with the risk of infarction (although to a lesser extent than with coronary atherosclerosis). Conclusion-ACE deletion polymorphism is strongly and independently associated with coronary atherosclerosis and, to a lesser extent, with myocardial infarction. As such, the results are analogous to what has already been reported in French white, Japanese, and Welsh coronary patients.

Original languageEnglish
Pages (from-to)584-591
Number of pages8
JournalHeart
Volume74
Issue number6
DOIs
Publication statusPublished - 1995

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Gene Deletion
Peptidyl-Dipeptidase A
Coronary Artery Disease
Alleles
Myocardial Infarction
Genotype
Odds Ratio
Hypertension
Blood Donors
Gene Frequency
Cohort Studies
Blood Group Antigens
Infarction
Italy
Atherosclerosis
Coronary Vessels
Leukocytes
Logistic Models
Outcome Assessment (Health Care)
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Gastroenterology
  • Cardiology and Cardiovascular Medicine

Cite this

Angiotensin converting enzyme gene deletion allele is independently and strongly associated with coronary atherosclerosis and myocardial infarction. / Arbustini, Eloisa; Grasso, Maurizia; Fasani, Roberta; Klersy, Catherine; Diegoli, Marta; Porcu, Emanuele; Banchieri, Nadia; Fortina, Paolo; Danesino, Cesare; Specchia, Giuseppe.

In: Heart, Vol. 74, No. 6, 1995, p. 584-591.

Research output: Contribution to journalArticle

Arbustini, Eloisa ; Grasso, Maurizia ; Fasani, Roberta ; Klersy, Catherine ; Diegoli, Marta ; Porcu, Emanuele ; Banchieri, Nadia ; Fortina, Paolo ; Danesino, Cesare ; Specchia, Giuseppe. / Angiotensin converting enzyme gene deletion allele is independently and strongly associated with coronary atherosclerosis and myocardial infarction. In: Heart. 1995 ; Vol. 74, No. 6. pp. 584-591.
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abstract = "Objective-To investigate the association of the three angiotensin converting enzyme (ACE) genotypes, DD, ID, and IH, with the occurrence or absence of coronary atherosclerosis and with myocardial infarction and hypertension. Design-Cohort analysis study. Setting-North-Italy reference centre. Subjects-388 white Italian patients (281 males; mean age 60-7 (SD 12-5) years) with proven coronary atherosclerosis (n = 255) or with angiographically normal coronary arteries (n = 133). A further group of 290 healthy blood donors was tested for allele frequency comparison. Interventions-ACEIID polymorphism was analysed with polymerase chain reaction on DNA from white blood cells. Main outcome measures-Coronary atherosclerosis, myocardial infarction, hypertension. Results-The D and I allele frequencies were respectively 0-63 and 0 37 in the overall healthy blood donor group and 0-66 and 0.34 in the overall study group. In the latter, univariate analysis showed (1) that coronary atherosclerosis (255 patients) was associated with the deletion allele, with an odds ratio (OR) of 5.78 for DDIII, P <0.001, and 2.39 for IDIII, P = 0-006; and (2) that myocardial infarction (154 patients) was associated with the DD genotype (OR DDIII = 2-56, P = 0.007), but not with the ID genotype (OR DDIII = 196, P = 0.056). Finally, hypertension proved to be unrelated with the ACE genotype. The distribution between the three genotypes of known risk factors for coronary artery disease was similar. Logistic regression modelling, performed to test the association of the selected risk factors simultaneously with coronary atherosclerosis and myocardial infarction, showed that the deletion allele (whether DD or ID) was the strongest risk factor for atherosclerosis, and that the D allele was significantly associated with the risk of infarction (although to a lesser extent than with coronary atherosclerosis). Conclusion-ACE deletion polymorphism is strongly and independently associated with coronary atherosclerosis and, to a lesser extent, with myocardial infarction. As such, the results are analogous to what has already been reported in French white, Japanese, and Welsh coronary patients.",
author = "Eloisa Arbustini and Maurizia Grasso and Roberta Fasani and Catherine Klersy and Marta Diegoli and Emanuele Porcu and Nadia Banchieri and Paolo Fortina and Cesare Danesino and Giuseppe Specchia",
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T1 - Angiotensin converting enzyme gene deletion allele is independently and strongly associated with coronary atherosclerosis and myocardial infarction

AU - Arbustini, Eloisa

AU - Grasso, Maurizia

AU - Fasani, Roberta

AU - Klersy, Catherine

AU - Diegoli, Marta

AU - Porcu, Emanuele

AU - Banchieri, Nadia

AU - Fortina, Paolo

AU - Danesino, Cesare

AU - Specchia, Giuseppe

PY - 1995

Y1 - 1995

N2 - Objective-To investigate the association of the three angiotensin converting enzyme (ACE) genotypes, DD, ID, and IH, with the occurrence or absence of coronary atherosclerosis and with myocardial infarction and hypertension. Design-Cohort analysis study. Setting-North-Italy reference centre. Subjects-388 white Italian patients (281 males; mean age 60-7 (SD 12-5) years) with proven coronary atherosclerosis (n = 255) or with angiographically normal coronary arteries (n = 133). A further group of 290 healthy blood donors was tested for allele frequency comparison. Interventions-ACEIID polymorphism was analysed with polymerase chain reaction on DNA from white blood cells. Main outcome measures-Coronary atherosclerosis, myocardial infarction, hypertension. Results-The D and I allele frequencies were respectively 0-63 and 0 37 in the overall healthy blood donor group and 0-66 and 0.34 in the overall study group. In the latter, univariate analysis showed (1) that coronary atherosclerosis (255 patients) was associated with the deletion allele, with an odds ratio (OR) of 5.78 for DDIII, P <0.001, and 2.39 for IDIII, P = 0-006; and (2) that myocardial infarction (154 patients) was associated with the DD genotype (OR DDIII = 2-56, P = 0.007), but not with the ID genotype (OR DDIII = 196, P = 0.056). Finally, hypertension proved to be unrelated with the ACE genotype. The distribution between the three genotypes of known risk factors for coronary artery disease was similar. Logistic regression modelling, performed to test the association of the selected risk factors simultaneously with coronary atherosclerosis and myocardial infarction, showed that the deletion allele (whether DD or ID) was the strongest risk factor for atherosclerosis, and that the D allele was significantly associated with the risk of infarction (although to a lesser extent than with coronary atherosclerosis). Conclusion-ACE deletion polymorphism is strongly and independently associated with coronary atherosclerosis and, to a lesser extent, with myocardial infarction. As such, the results are analogous to what has already been reported in French white, Japanese, and Welsh coronary patients.

AB - Objective-To investigate the association of the three angiotensin converting enzyme (ACE) genotypes, DD, ID, and IH, with the occurrence or absence of coronary atherosclerosis and with myocardial infarction and hypertension. Design-Cohort analysis study. Setting-North-Italy reference centre. Subjects-388 white Italian patients (281 males; mean age 60-7 (SD 12-5) years) with proven coronary atherosclerosis (n = 255) or with angiographically normal coronary arteries (n = 133). A further group of 290 healthy blood donors was tested for allele frequency comparison. Interventions-ACEIID polymorphism was analysed with polymerase chain reaction on DNA from white blood cells. Main outcome measures-Coronary atherosclerosis, myocardial infarction, hypertension. Results-The D and I allele frequencies were respectively 0-63 and 0 37 in the overall healthy blood donor group and 0-66 and 0.34 in the overall study group. In the latter, univariate analysis showed (1) that coronary atherosclerosis (255 patients) was associated with the deletion allele, with an odds ratio (OR) of 5.78 for DDIII, P <0.001, and 2.39 for IDIII, P = 0-006; and (2) that myocardial infarction (154 patients) was associated with the DD genotype (OR DDIII = 2-56, P = 0.007), but not with the ID genotype (OR DDIII = 196, P = 0.056). Finally, hypertension proved to be unrelated with the ACE genotype. The distribution between the three genotypes of known risk factors for coronary artery disease was similar. Logistic regression modelling, performed to test the association of the selected risk factors simultaneously with coronary atherosclerosis and myocardial infarction, showed that the deletion allele (whether DD or ID) was the strongest risk factor for atherosclerosis, and that the D allele was significantly associated with the risk of infarction (although to a lesser extent than with coronary atherosclerosis). Conclusion-ACE deletion polymorphism is strongly and independently associated with coronary atherosclerosis and, to a lesser extent, with myocardial infarction. As such, the results are analogous to what has already been reported in French white, Japanese, and Welsh coronary patients.

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