Angiotensin II directly stimulates release of atrial natriuretic factor in isolated rabbit hearts

A. Focaccio, M. Volpe, G. Ambrosio, G. Lembo, S. Pannain, S. Rubattu, I. Enea, S. Pignalosa, M. Chiariello

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Background. Previous studies have shown that infusion of angiotensin II (Ang II) increases plasma concentrations of atrial natriuretic factor (ANF) in vivo. This phenomenon has been considered secondary to the effects of Ang II on cardiac and systemic hemodynamics. The present study was designed to assess whether Ang II may exert a direct stimulatory effect on ANF release from the heart independent of changes in hemodynamics. Methods and Results. Isolated rabbit hearts were perfused in the Langendorff mode. Heart rate, coronary flow, and atrial and left ventricular (LV) volumes were kept constant. After stabilization, Ang II was infused intracoronary at increasing doses (10-11 to 10-8 M) in nine hearts and at a single dose of 10-10 M in 10 hearts. Each infusion lasted for 5 minutes and was followed by a 10- minute washout period. Four hearts received vehicle alone for 80 minutes. Ang II induced a dose-dependent increase in coronary perfusion pressure and in LV developed pressure. ANF release, measured by radioimmunoassay on the extracts of the cardiac effluent, also increased during Ang II infusion and returned to the basal values during the 10-minute washout period. In the control group, coronary perfusion pressure, LV developed pressure, and LV end- diastolic pressure did not change appreciably over the observation period, whereas ANF release progressively decreased during perfusion. Conclusions. Ang II can directly stimulate cardiac release of ANF in isolated rabbit hearts independently of changes in hemodynamics.

Original languageEnglish
Pages (from-to)192-198
Number of pages7
JournalCirculation
Volume87
Issue number1
Publication statusPublished - 1993

Fingerprint

Atrial Natriuretic Factor
Angiotensin II
Rabbits
Perfusion
Hemodynamics
Ventricular Pressure
Pressure
Radioimmunoassay
Heart Rate
Observation
Blood Pressure
Control Groups

Keywords

  • angiotensin
  • atrial natriuretic factor

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Angiotensin II directly stimulates release of atrial natriuretic factor in isolated rabbit hearts. / Focaccio, A.; Volpe, M.; Ambrosio, G.; Lembo, G.; Pannain, S.; Rubattu, S.; Enea, I.; Pignalosa, S.; Chiariello, M.

In: Circulation, Vol. 87, No. 1, 1993, p. 192-198.

Research output: Contribution to journalArticle

Focaccio, A, Volpe, M, Ambrosio, G, Lembo, G, Pannain, S, Rubattu, S, Enea, I, Pignalosa, S & Chiariello, M 1993, 'Angiotensin II directly stimulates release of atrial natriuretic factor in isolated rabbit hearts', Circulation, vol. 87, no. 1, pp. 192-198.
Focaccio, A. ; Volpe, M. ; Ambrosio, G. ; Lembo, G. ; Pannain, S. ; Rubattu, S. ; Enea, I. ; Pignalosa, S. ; Chiariello, M. / Angiotensin II directly stimulates release of atrial natriuretic factor in isolated rabbit hearts. In: Circulation. 1993 ; Vol. 87, No. 1. pp. 192-198.
@article{dc875ea63e454800b12d9c19ccc35ad0,
title = "Angiotensin II directly stimulates release of atrial natriuretic factor in isolated rabbit hearts",
abstract = "Background. Previous studies have shown that infusion of angiotensin II (Ang II) increases plasma concentrations of atrial natriuretic factor (ANF) in vivo. This phenomenon has been considered secondary to the effects of Ang II on cardiac and systemic hemodynamics. The present study was designed to assess whether Ang II may exert a direct stimulatory effect on ANF release from the heart independent of changes in hemodynamics. Methods and Results. Isolated rabbit hearts were perfused in the Langendorff mode. Heart rate, coronary flow, and atrial and left ventricular (LV) volumes were kept constant. After stabilization, Ang II was infused intracoronary at increasing doses (10-11 to 10-8 M) in nine hearts and at a single dose of 10-10 M in 10 hearts. Each infusion lasted for 5 minutes and was followed by a 10- minute washout period. Four hearts received vehicle alone for 80 minutes. Ang II induced a dose-dependent increase in coronary perfusion pressure and in LV developed pressure. ANF release, measured by radioimmunoassay on the extracts of the cardiac effluent, also increased during Ang II infusion and returned to the basal values during the 10-minute washout period. In the control group, coronary perfusion pressure, LV developed pressure, and LV end- diastolic pressure did not change appreciably over the observation period, whereas ANF release progressively decreased during perfusion. Conclusions. Ang II can directly stimulate cardiac release of ANF in isolated rabbit hearts independently of changes in hemodynamics.",
keywords = "angiotensin, atrial natriuretic factor",
author = "A. Focaccio and M. Volpe and G. Ambrosio and G. Lembo and S. Pannain and S. Rubattu and I. Enea and S. Pignalosa and M. Chiariello",
year = "1993",
language = "English",
volume = "87",
pages = "192--198",
journal = "Circulation",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Angiotensin II directly stimulates release of atrial natriuretic factor in isolated rabbit hearts

AU - Focaccio, A.

AU - Volpe, M.

AU - Ambrosio, G.

AU - Lembo, G.

AU - Pannain, S.

AU - Rubattu, S.

AU - Enea, I.

AU - Pignalosa, S.

AU - Chiariello, M.

PY - 1993

Y1 - 1993

N2 - Background. Previous studies have shown that infusion of angiotensin II (Ang II) increases plasma concentrations of atrial natriuretic factor (ANF) in vivo. This phenomenon has been considered secondary to the effects of Ang II on cardiac and systemic hemodynamics. The present study was designed to assess whether Ang II may exert a direct stimulatory effect on ANF release from the heart independent of changes in hemodynamics. Methods and Results. Isolated rabbit hearts were perfused in the Langendorff mode. Heart rate, coronary flow, and atrial and left ventricular (LV) volumes were kept constant. After stabilization, Ang II was infused intracoronary at increasing doses (10-11 to 10-8 M) in nine hearts and at a single dose of 10-10 M in 10 hearts. Each infusion lasted for 5 minutes and was followed by a 10- minute washout period. Four hearts received vehicle alone for 80 minutes. Ang II induced a dose-dependent increase in coronary perfusion pressure and in LV developed pressure. ANF release, measured by radioimmunoassay on the extracts of the cardiac effluent, also increased during Ang II infusion and returned to the basal values during the 10-minute washout period. In the control group, coronary perfusion pressure, LV developed pressure, and LV end- diastolic pressure did not change appreciably over the observation period, whereas ANF release progressively decreased during perfusion. Conclusions. Ang II can directly stimulate cardiac release of ANF in isolated rabbit hearts independently of changes in hemodynamics.

AB - Background. Previous studies have shown that infusion of angiotensin II (Ang II) increases plasma concentrations of atrial natriuretic factor (ANF) in vivo. This phenomenon has been considered secondary to the effects of Ang II on cardiac and systemic hemodynamics. The present study was designed to assess whether Ang II may exert a direct stimulatory effect on ANF release from the heart independent of changes in hemodynamics. Methods and Results. Isolated rabbit hearts were perfused in the Langendorff mode. Heart rate, coronary flow, and atrial and left ventricular (LV) volumes were kept constant. After stabilization, Ang II was infused intracoronary at increasing doses (10-11 to 10-8 M) in nine hearts and at a single dose of 10-10 M in 10 hearts. Each infusion lasted for 5 minutes and was followed by a 10- minute washout period. Four hearts received vehicle alone for 80 minutes. Ang II induced a dose-dependent increase in coronary perfusion pressure and in LV developed pressure. ANF release, measured by radioimmunoassay on the extracts of the cardiac effluent, also increased during Ang II infusion and returned to the basal values during the 10-minute washout period. In the control group, coronary perfusion pressure, LV developed pressure, and LV end- diastolic pressure did not change appreciably over the observation period, whereas ANF release progressively decreased during perfusion. Conclusions. Ang II can directly stimulate cardiac release of ANF in isolated rabbit hearts independently of changes in hemodynamics.

KW - angiotensin

KW - atrial natriuretic factor

UR - http://www.scopus.com/inward/record.url?scp=0027524135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027524135&partnerID=8YFLogxK

M3 - Article

VL - 87

SP - 192

EP - 198

JO - Circulation

JF - Circulation

SN - 0009-7322

IS - 1

ER -