Background - We evaluated whether angiotensin II (Ang II) influenced intercellular adhesion molecule (ICAM)-1 expression by human vascular endothelial cells derived from umbilical cord veins (HUVECs) and plasma soluble ICAM-1 levels in vivo. Methods and Results - Cultured HUVECs were incubated with Ang II (from 10-9 to 10-6 mol/L) with or without candesartan and PD12319 (inhibitors of Ang II AT1 and AT2 receptors, respectively) for various times up to 4 hours. Total RNA was then extracted from HUVECs, and Northern blots were probed with a 1.9-kb ICAM-1 cDNA fragment. HUVEC supernatants were used to assess soluble ICAM-1 release by ELISA. Northern blot analysis detected a strong increase of ICAM-1 mRNA after 2-hour incubation with Ang II. The response was inhibited by candesartan. Soluble ICAM-1 release by HUVECs also increased (P-1 · min- 1, to be increased each 30 minutes by 2.0 ng · kg-1 · min-1 to the final rate of 7.0 ng · kg-1 · min-1) was infused in 8 normotensive and 12 essential hypertensive individuals. In the latter, Ang II was reinfused after 4 weeks on either placebo (n=3), losartan (50 mg UID, n=5), or atenolol (50 mg UID, n=4) treatment. Plasma soluble ICAM-1 levels increased after Ang II infusion in hypertensives and normotensives (P1 receptor blockade inhibits such endothelial effects of Ang II.
|Number of pages||7|
|Publication status||Published - Oct 12 1999|
- Cell adhesion molecules
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine