Angiotensin II stimulates intercellular adhesion molecule-1 (ICAM-1) expression by human vascular endothelial cells and increases soluble ICAM-1 release in vivo

L. Pastore, A. Tessitore, S. Martinotti, E. Toniato, E. Alesse, M. C. Bravi, Claudio Ferri, G. Desideri, A. Gulino, A. Santucci

Research output: Contribution to journalArticle

Abstract

Background - We evaluated whether angiotensin II (Ang II) influenced intercellular adhesion molecule (ICAM)-1 expression by human vascular endothelial cells derived from umbilical cord veins (HUVECs) and plasma soluble ICAM-1 levels in vivo. Methods and Results - Cultured HUVECs were incubated with Ang II (from 10-9 to 10-6 mol/L) with or without candesartan and PD12319 (inhibitors of Ang II AT1 and AT2 receptors, respectively) for various times up to 4 hours. Total RNA was then extracted from HUVECs, and Northern blots were probed with a 1.9-kb ICAM-1 cDNA fragment. HUVEC supernatants were used to assess soluble ICAM-1 release by ELISA. Northern blot analysis detected a strong increase of ICAM-1 mRNA after 2-hour incubation with Ang II. The response was inhibited by candesartan. Soluble ICAM-1 release by HUVECs also increased (P-1 · min- 1, to be increased each 30 minutes by 2.0 ng · kg-1 · min-1 to the final rate of 7.0 ng · kg-1 · min-1) was infused in 8 normotensive and 12 essential hypertensive individuals. In the latter, Ang II was reinfused after 4 weeks on either placebo (n=3), losartan (50 mg UID, n=5), or atenolol (50 mg UID, n=4) treatment. Plasma soluble ICAM-1 levels increased after Ang II infusion in hypertensives and normotensives (P1 receptor blockade inhibits such endothelial effects of Ang II.

Original languageEnglish
Pages (from-to)1646-1652
Number of pages7
JournalCirculation
Volume100
Issue number15
Publication statusPublished - Oct 12 1999

Fingerprint

Intercellular Adhesion Molecule-1
Angiotensin II
Endothelial Cells
Northern Blotting
Angiotensin Type 2 Receptor
Angiotensin Type 1 Receptor
Umbilical Veins
Angiotensin Receptors
Atenolol
Losartan
Umbilical Cord
Complementary DNA
Enzyme-Linked Immunosorbent Assay
Placebos
RNA
Messenger RNA

Keywords

  • Angiotensin
  • Cell adhesion molecules
  • Cells
  • Endothelium

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Pastore, L., Tessitore, A., Martinotti, S., Toniato, E., Alesse, E., Bravi, M. C., ... Santucci, A. (1999). Angiotensin II stimulates intercellular adhesion molecule-1 (ICAM-1) expression by human vascular endothelial cells and increases soluble ICAM-1 release in vivo. Circulation, 100(15), 1646-1652.

Angiotensin II stimulates intercellular adhesion molecule-1 (ICAM-1) expression by human vascular endothelial cells and increases soluble ICAM-1 release in vivo. / Pastore, L.; Tessitore, A.; Martinotti, S.; Toniato, E.; Alesse, E.; Bravi, M. C.; Ferri, Claudio; Desideri, G.; Gulino, A.; Santucci, A.

In: Circulation, Vol. 100, No. 15, 12.10.1999, p. 1646-1652.

Research output: Contribution to journalArticle

Pastore, L, Tessitore, A, Martinotti, S, Toniato, E, Alesse, E, Bravi, MC, Ferri, C, Desideri, G, Gulino, A & Santucci, A 1999, 'Angiotensin II stimulates intercellular adhesion molecule-1 (ICAM-1) expression by human vascular endothelial cells and increases soluble ICAM-1 release in vivo', Circulation, vol. 100, no. 15, pp. 1646-1652.
Pastore, L. ; Tessitore, A. ; Martinotti, S. ; Toniato, E. ; Alesse, E. ; Bravi, M. C. ; Ferri, Claudio ; Desideri, G. ; Gulino, A. ; Santucci, A. / Angiotensin II stimulates intercellular adhesion molecule-1 (ICAM-1) expression by human vascular endothelial cells and increases soluble ICAM-1 release in vivo. In: Circulation. 1999 ; Vol. 100, No. 15. pp. 1646-1652.
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AU - Pastore, L.

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AU - Martinotti, S.

AU - Toniato, E.

AU - Alesse, E.

AU - Bravi, M. C.

AU - Ferri, Claudio

AU - Desideri, G.

AU - Gulino, A.

AU - Santucci, A.

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N2 - Background - We evaluated whether angiotensin II (Ang II) influenced intercellular adhesion molecule (ICAM)-1 expression by human vascular endothelial cells derived from umbilical cord veins (HUVECs) and plasma soluble ICAM-1 levels in vivo. Methods and Results - Cultured HUVECs were incubated with Ang II (from 10-9 to 10-6 mol/L) with or without candesartan and PD12319 (inhibitors of Ang II AT1 and AT2 receptors, respectively) for various times up to 4 hours. Total RNA was then extracted from HUVECs, and Northern blots were probed with a 1.9-kb ICAM-1 cDNA fragment. HUVEC supernatants were used to assess soluble ICAM-1 release by ELISA. Northern blot analysis detected a strong increase of ICAM-1 mRNA after 2-hour incubation with Ang II. The response was inhibited by candesartan. Soluble ICAM-1 release by HUVECs also increased (P-1 · min- 1, to be increased each 30 minutes by 2.0 ng · kg-1 · min-1 to the final rate of 7.0 ng · kg-1 · min-1) was infused in 8 normotensive and 12 essential hypertensive individuals. In the latter, Ang II was reinfused after 4 weeks on either placebo (n=3), losartan (50 mg UID, n=5), or atenolol (50 mg UID, n=4) treatment. Plasma soluble ICAM-1 levels increased after Ang II infusion in hypertensives and normotensives (P1 receptor blockade inhibits such endothelial effects of Ang II.

AB - Background - We evaluated whether angiotensin II (Ang II) influenced intercellular adhesion molecule (ICAM)-1 expression by human vascular endothelial cells derived from umbilical cord veins (HUVECs) and plasma soluble ICAM-1 levels in vivo. Methods and Results - Cultured HUVECs were incubated with Ang II (from 10-9 to 10-6 mol/L) with or without candesartan and PD12319 (inhibitors of Ang II AT1 and AT2 receptors, respectively) for various times up to 4 hours. Total RNA was then extracted from HUVECs, and Northern blots were probed with a 1.9-kb ICAM-1 cDNA fragment. HUVEC supernatants were used to assess soluble ICAM-1 release by ELISA. Northern blot analysis detected a strong increase of ICAM-1 mRNA after 2-hour incubation with Ang II. The response was inhibited by candesartan. Soluble ICAM-1 release by HUVECs also increased (P-1 · min- 1, to be increased each 30 minutes by 2.0 ng · kg-1 · min-1 to the final rate of 7.0 ng · kg-1 · min-1) was infused in 8 normotensive and 12 essential hypertensive individuals. In the latter, Ang II was reinfused after 4 weeks on either placebo (n=3), losartan (50 mg UID, n=5), or atenolol (50 mg UID, n=4) treatment. Plasma soluble ICAM-1 levels increased after Ang II infusion in hypertensives and normotensives (P1 receptor blockade inhibits such endothelial effects of Ang II.

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