TY - CHAP
T1 - Animal models of multiple sclerosis
AU - Furlan, Roberto
AU - Cuomo, Carmela
AU - Martino, Gianvito
PY - 2009
Y1 - 2009
N2 - Since its first description, experimental autoimmune encephalomyelitis, originally designated experimental allergic encephalitis (EAE), has been proposed as animal model to investigate pathogenetic hypotheses and test new treatments in the field of central nervous system inflammation and demyelination, which has become, in the last 30 years, the most popular animal model of multiple sclerosis (MS). This experimental disease can be obtained in all mammals tested so far, including nonhuman primates, allowing very advanced preclinical studies. Its appropriate use has led to the development of the most recent treatments approved for MS, also demonstrating its predictive value when properly handled. Some of the most exciting experiments validating the use of neural precursor cells (NPCs) as a potential therapeutic option in CNS inflammation have been performed in this model. We review here the most relevant immunological features of EAE in the different animal species and strains, and describe detailed protocols to obtain the three most common clinical courses of EAE in mice, with the hope to provide both cultural and practical basis for the use of this fascinating animal model.
AB - Since its first description, experimental autoimmune encephalomyelitis, originally designated experimental allergic encephalitis (EAE), has been proposed as animal model to investigate pathogenetic hypotheses and test new treatments in the field of central nervous system inflammation and demyelination, which has become, in the last 30 years, the most popular animal model of multiple sclerosis (MS). This experimental disease can be obtained in all mammals tested so far, including nonhuman primates, allowing very advanced preclinical studies. Its appropriate use has led to the development of the most recent treatments approved for MS, also demonstrating its predictive value when properly handled. Some of the most exciting experiments validating the use of neural precursor cells (NPCs) as a potential therapeutic option in CNS inflammation have been performed in this model. We review here the most relevant immunological features of EAE in the different animal species and strains, and describe detailed protocols to obtain the three most common clinical courses of EAE in mice, with the hope to provide both cultural and practical basis for the use of this fascinating animal model.
KW - Disease induction
KW - Experimental autoimmune encephalomyelitis
KW - Multiple sclerosis
UR - http://www.scopus.com/inward/record.url?scp=67649342797&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67649342797&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-931-4_11
DO - 10.1007/978-1-60327-931-4_11
M3 - Chapter
C2 - 19378202
AN - SCOPUS:67649342797
SN - 9781603279307
VL - 549
T3 - Methods in Molecular Biology
SP - 157
EP - 173
BT - Methods in Molecular Biology
ER -