Anodal transcranial direct current stimulation boosts synaptic plasticity and memory in mice via epigenetic regulation of Bdnf expression

Maria Vittoria Podda, Sara Cocco, Alessia Mastrodonato, Salvatore Fusco, Lucia Leone, Saviana Antonella Barbati, Claudia Colussi, Cristian Ripoli, Claudio Grassi

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The effects of transcranial direct current stimulation (tDCS) on brain functions and the underlying molecular mechanisms are yet largely unknown. Here we report that mice subjected to 20-min anodal tDCS exhibited one-week lasting increases in hippocampal LTP, learning and memory. These effects were associated with enhanced: i) acetylation of brain-derived neurotrophic factor (Bdnf) promoter I; ii) expression of Bdnf exons I and IX; iii) Bdnf protein levels. The hippocampi of stimulated mice also exhibited enhanced CREB phosphorylation, pCREB binding to Bdnf promoter I and recruitment of CBP on the same regulatory sequence. Inhibition of acetylation and blockade of TrkB receptors hindered tDCS effects at molecular, electrophysiological and behavioral levels. Collectively, our findings suggest that anodal tDCS increases hippocampal LTP and memory via chromatin remodeling of Bdnf regulatory sequences leading to increased expression of this gene, and support the therapeutic potential of tDCS for brain diseases associated with impaired neuroplasticity.

Original languageEnglish
Article number22180
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Feb 24 2016

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Neuronal Plasticity
Brain-Derived Neurotrophic Factor
Epigenomics
Acetylation
trkB Receptor
Factor IX
Chromatin Assembly and Disassembly
Nerve Growth Factors
Brain Diseases
Exons
Hippocampus
Phosphorylation
Transcranial Direct Current Stimulation
Learning
Gene Expression
Brain

ASJC Scopus subject areas

  • General

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Anodal transcranial direct current stimulation boosts synaptic plasticity and memory in mice via epigenetic regulation of Bdnf expression. / Podda, Maria Vittoria; Cocco, Sara; Mastrodonato, Alessia; Fusco, Salvatore; Leone, Lucia; Barbati, Saviana Antonella; Colussi, Claudia; Ripoli, Cristian; Grassi, Claudio.

In: Scientific Reports, Vol. 6, 22180, 24.02.2016.

Research output: Contribution to journalArticle

Podda, Maria Vittoria ; Cocco, Sara ; Mastrodonato, Alessia ; Fusco, Salvatore ; Leone, Lucia ; Barbati, Saviana Antonella ; Colussi, Claudia ; Ripoli, Cristian ; Grassi, Claudio. / Anodal transcranial direct current stimulation boosts synaptic plasticity and memory in mice via epigenetic regulation of Bdnf expression. In: Scientific Reports. 2016 ; Vol. 6.
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