Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells

Gennaro Di Maro, Paolo Salerno, Kristian Unger, Francesca M. Orlandella, Mario Monaco, Gennaro Chiappetta, Gerry Thomas, Malgorzata Oczko-Wojciechowska, Mariorosario Masullo, Barbara Jarzab, Massimo Santoro, Giuliana Salvatore

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: Through a transcriptome microarray analysis, we have isolated Anterior gradient protein 2 (AGR2) as a gene up-regulated in papillary thyroid carcinoma (PTC). AGR2 is a disulfide isomerase over-expressed in several human carcinomas and recently linked to endoplasmic reticulum (ER) stress. Here, we analyzed the expression of AGR2 in PTC and its functional role.Methods: Expression of AGR2 was studied by immunohistochemistry and real time PCR in normal thyroids and in PTC samples. The function of AGR2 was studied by knockdown in PTC cells and by ectopic expression in non-transformed thyroid cells. The role of AGR2 in the ER stress was analyzed upon treatment of cells, expressing or not AGR2, with Bortezomib and analyzing by Western blot the expression levels of GADD153.Results: PTC over-expressed AGR2 at mRNA and protein levels. Knockdown of AGR2 in PTC cells induced apoptosis and decreased migration and invasion. Ectopic expression of AGR2 in non-transformed human thyroid cells increased migration and invasion and protected cells from ER stress induced by Bortezomib.Conclusions: AGR2 is a novel marker of PTC and plays a role in thyroid cancer cell survival, migration, invasion and protection from ER stress.

Original languageEnglish
Article number160
JournalMolecular Cancer
Volume13
Issue number1
DOIs
Publication statusPublished - Jun 30 2014

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Survival
Proteins
Endoplasmic Reticulum Stress
Thyroid Gland
Cell Movement
Papillary Thyroid cancer
Protein Disulfide-Isomerases
Cytoprotection
Gene Expression Profiling
Microarray Analysis
Thyroid Neoplasms
Real-Time Polymerase Chain Reaction
Cell Survival
Western Blotting
Immunohistochemistry
Apoptosis
Carcinoma
Messenger RNA
Genes

Keywords

  • AGR2
  • Endoplasmic reticulum stress
  • Migration and invasion
  • Survival
  • Thyroid cancer

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Oncology

Cite this

Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells. / Di Maro, Gennaro; Salerno, Paolo; Unger, Kristian; Orlandella, Francesca M.; Monaco, Mario; Chiappetta, Gennaro; Thomas, Gerry; Oczko-Wojciechowska, Malgorzata; Masullo, Mariorosario; Jarzab, Barbara; Santoro, Massimo; Salvatore, Giuliana.

In: Molecular Cancer, Vol. 13, No. 1, 160, 30.06.2014.

Research output: Contribution to journalArticle

Di Maro, G, Salerno, P, Unger, K, Orlandella, FM, Monaco, M, Chiappetta, G, Thomas, G, Oczko-Wojciechowska, M, Masullo, M, Jarzab, B, Santoro, M & Salvatore, G 2014, 'Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells', Molecular Cancer, vol. 13, no. 1, 160. https://doi.org/10.1186/1476-4598-13-160
Di Maro, Gennaro ; Salerno, Paolo ; Unger, Kristian ; Orlandella, Francesca M. ; Monaco, Mario ; Chiappetta, Gennaro ; Thomas, Gerry ; Oczko-Wojciechowska, Malgorzata ; Masullo, Mariorosario ; Jarzab, Barbara ; Santoro, Massimo ; Salvatore, Giuliana. / Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells. In: Molecular Cancer. 2014 ; Vol. 13, No. 1.
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AU - Monaco, Mario

AU - Chiappetta, Gennaro

AU - Thomas, Gerry

AU - Oczko-Wojciechowska, Malgorzata

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