[Anti-β2 Glycoprotein I - β2 Glycoprotein I] immune complexes in patients with antiphospholipid syndrome and other autoimmune diseases

A. Biasiolo, P. Rampazzo, T. Brocco, F. Barbero, A. Rosato, V. Pengo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Antiphospholipid syndrome (APS) is defined by the presence of aPL antibodies in patients with thromboembolic phenomena. Some antiphospholipid (aPL) antibodies, such as those directed against β2-glycoprotein I (β2GPI), are associated with thromboembolism, possess Lupus Anticoagulant (LA) activity and recognize their target antigen only when bound to specific surfaces or to phospholipids (PL). To ascertain whether both free and antibody-bound β2GPI circulate in APS, we set up an ELISA to detect [IgG anti-β2GPI-β2GPI] immune complexes. In this system, rabbit anti-human β2GPI antibodies were adsorbed onto plastic plates, incubated with patient plasma, and bound complexes were detected by means of alkaline phosphatase-labeled goat anti-human IgG; each assay was stopped when positive controls consisting of in vitro generated immune complexes reached an Optical Density (OD) of 0.5 at 405 nm. Plasma from 16 patients with APS showed a mean OD405 of 0.291 (range 0.115-0.558), not statistically different from the mean obtained for 15 age- and sex-matched healthy volunteers (mean OD405 = 0.169, range 0.066-0.264). Surprisingly, levels of immune complexes in 14 patients with other autoimmune diseases and no circulating anti-β2GPI antibodies were statistically higher (mean OD405 = 0.552, range 0.204-0.991) than those of healthy subjects and patients with APS. These data indicate that while autoantibodies to β2GPI are mainly unbound in plasma of patients with APS, they are complexed with their antigen in patients with other autoimmune diseases, possibly reflecting a higher binding affinity.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalLupus
Volume8
Issue number2
Publication statusPublished - 1999

Fingerprint

Antiphospholipid Syndrome
Antigen-Antibody Complex
Autoimmune Diseases
Glycoproteins
Antiphospholipid Antibodies
Antibodies
Healthy Volunteers
Antigens
Lupus Coagulation Inhibitor
Thromboembolism
Goats
Autoantibodies
Plastics
Alkaline Phosphatase
Phospholipids
Enzyme-Linked Immunosorbent Assay
Rabbits

Keywords

  • Anti-β-glycoprotein I
  • Antiphospholipid syndrome
  • Immune complexes

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

[Anti-β2 Glycoprotein I - β2 Glycoprotein I] immune complexes in patients with antiphospholipid syndrome and other autoimmune diseases. / Biasiolo, A.; Rampazzo, P.; Brocco, T.; Barbero, F.; Rosato, A.; Pengo, V.

In: Lupus, Vol. 8, No. 2, 1999, p. 121-126.

Research output: Contribution to journalArticle

Biasiolo, A. ; Rampazzo, P. ; Brocco, T. ; Barbero, F. ; Rosato, A. ; Pengo, V. / [Anti-β2 Glycoprotein I - β2 Glycoprotein I] immune complexes in patients with antiphospholipid syndrome and other autoimmune diseases. In: Lupus. 1999 ; Vol. 8, No. 2. pp. 121-126.
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