Anti-bacterial effect of essential oil from Xanthium strumarium against shiga toxin-producing Escherichia coli

J. Sharifi-Rad, L. Soufi, S. A M Ayatollahi, Marcello Iriti, M. Sharifi-Rad, Elena Maria Varoni, F. Shahri, Susanna Esposito, K. Kuhestani, M. Sharifi-Rad

Research output: Contribution to journalArticle

Abstract

Shiga toxin-producing Escherichia coli (STEC) serotype O157:H7 is one of the most important human pathogenic microorganisms, which can cause life-threatening infections. Xanthium strumarium L. is a plant with anti-bacterial activity against gram-negative and gram-positive bacteria. This study aims to demonstrate in vitro efficacy of the essential oil (EO) extracted from Xanthium strumarium L. against E. coli O157:H7. Using the agar test diffusion, the effect of Xanthium strumarium L. EO (5, 10, 15, 30, 60, and 120 mg/mL) was verified at each of the four different growth phases of E. coli O157:H7. Cell counts of viable cells and colony forming unit (CFU) were determined at regular time points using Breed's method and colony counting method, respectively. No viable cell was detectable after the 1 hour-exposure to X. strumarium EO at 30, 60, and 120 mg/mL concentrations. No bacterial colony was formed after 1 h until the end of the incubation period at 24 h. At lower concentrations, the number of bacteria cells decreased and colonies could be observed only after incubation. At the exponential phase, the EO at 15 mg/mL was only bacteriostatic, while from 30 mg/mL started to be bactericidal. X. strumarium EO antibacterial activity against Shiga toxin-producing E. coli O157:H7 is dependent on EO concentration and physiological state of the microorganisms tested. The best inhibitory activity was achieved during the late exponential and the stationary phases.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalCellular and Molecular Biology
Volume62
Issue number9
DOIs
Publication statusPublished - 2016

Fingerprint

Xanthium
Shiga Toxin
Shiga-Toxigenic Escherichia coli
Volatile Oils
Escherichia coli
Escherichia coli O157
Microorganisms
Bacteria
Cell Count
Gram-Positive Bacteria
Agar
Stem Cells
Growth
Infection

Keywords

  • Agar diffusion test
  • Medicinal Plant
  • Shiga toxin-producing E. coli (STEC)
  • Xanthium strumarium L.

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sharifi-Rad, J., Soufi, L., Ayatollahi, S. A. M., Iriti, M., Sharifi-Rad, M., Varoni, E. M., ... Sharifi-Rad, M. (2016). Anti-bacterial effect of essential oil from Xanthium strumarium against shiga toxin-producing Escherichia coli. Cellular and Molecular Biology, 62(9), 69-74. https://doi.org/10.14715/cmb/2016.62.9.11

Anti-bacterial effect of essential oil from Xanthium strumarium against shiga toxin-producing Escherichia coli. / Sharifi-Rad, J.; Soufi, L.; Ayatollahi, S. A M; Iriti, Marcello; Sharifi-Rad, M.; Varoni, Elena Maria; Shahri, F.; Esposito, Susanna; Kuhestani, K.; Sharifi-Rad, M.

In: Cellular and Molecular Biology, Vol. 62, No. 9, 2016, p. 69-74.

Research output: Contribution to journalArticle

Sharifi-Rad, J, Soufi, L, Ayatollahi, SAM, Iriti, M, Sharifi-Rad, M, Varoni, EM, Shahri, F, Esposito, S, Kuhestani, K & Sharifi-Rad, M 2016, 'Anti-bacterial effect of essential oil from Xanthium strumarium against shiga toxin-producing Escherichia coli', Cellular and Molecular Biology, vol. 62, no. 9, pp. 69-74. https://doi.org/10.14715/cmb/2016.62.9.11
Sharifi-Rad, J. ; Soufi, L. ; Ayatollahi, S. A M ; Iriti, Marcello ; Sharifi-Rad, M. ; Varoni, Elena Maria ; Shahri, F. ; Esposito, Susanna ; Kuhestani, K. ; Sharifi-Rad, M. / Anti-bacterial effect of essential oil from Xanthium strumarium against shiga toxin-producing Escherichia coli. In: Cellular and Molecular Biology. 2016 ; Vol. 62, No. 9. pp. 69-74.
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