Anti-cancer activity of plant-produced HPV16 E7 vaccine

Silvia Massa; Rosella Franconi; Rossella Brandi; Antonio Muller; Vadim Mett; Vidadi Yusibov; Aldo Venuti

doi:10.1016/j.vaccine.2007.01.018

Anti-cancer activity of plant-produced HPV16 E7 vaccine

Silvia Massa, Rosella Franconi, Rossella Brandi, Antonio Muller, Vadim Mett, Vidadi Yusibov, Aldo Venuti

Istituto Regina Elena

Research output: Contribution to journal › Article › peer-review

Abstract

The E7 oncoprotein from Human Papilloma Virus (HPV) is an attractive candidate for anti-cancer vaccine development. In this study, we engineered HPV16 E7 coding sequence (wild type or mutagenized sequence, E7GGG) as fusions to β-1,3-1,4-glucanase (LicKM) of Clostridium thermocellum and produced in Nicotiana benthamiana plants using a transient expression system. Target antigens were purified and evaluated in mice for their potential as prophylactic and therapeutic vaccine candidates. Both fusion proteins induced E7-specific IgG and cytotoxic T-cell responses and protected mice against challenge with E7-expressing tumor cells. Furthermore, when administered after challenge, these plant-produced antigens prevented tumor development.

Original language	English
Pages (from-to)	3018-3021
Number of pages	4
Journal	Vaccine
Volume	25
Issue number	16 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.vaccine.2007.01.018
Publication status	Published - Apr 20 2007

Keywords

Cancer vaccine
Human papilloma virus
Plant-produced vaccine

ASJC Scopus subject areas

Immunology
Microbiology
Virology
Infectious Diseases
Public Health, Environmental and Occupational Health
veterinary(all)

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10.1016/j.vaccine.2007.01.018

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title = "Anti-cancer activity of plant-produced HPV16 E7 vaccine",

abstract = "The E7 oncoprotein from Human Papilloma Virus (HPV) is an attractive candidate for anti-cancer vaccine development. In this study, we engineered HPV16 E7 coding sequence (wild type or mutagenized sequence, E7GGG) as fusions to β-1,3-1,4-glucanase (LicKM) of Clostridium thermocellum and produced in Nicotiana benthamiana plants using a transient expression system. Target antigens were purified and evaluated in mice for their potential as prophylactic and therapeutic vaccine candidates. Both fusion proteins induced E7-specific IgG and cytotoxic T-cell responses and protected mice against challenge with E7-expressing tumor cells. Furthermore, when administered after challenge, these plant-produced antigens prevented tumor development.",

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AU - Massa, Silvia

AU - Franconi, Rosella

AU - Brandi, Rossella

AU - Muller, Antonio

AU - Mett, Vadim

AU - Yusibov, Vidadi

AU - Venuti, Aldo

PY - 2007/4/20

Y1 - 2007/4/20

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KW - Human papilloma virus

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Anti-cancer activity of plant-produced HPV16 E7 vaccine

Abstract

Keywords

ASJC Scopus subject areas

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