Abstract
Anti-citrullinated protein antibodies (ACPA) are important serological markers in the diagnosis of rheumatoid arthritis (RA) and are part of the recent disease classification criteria. However, there is a strong need for reliable markers for measuring and predicting joint damage and disease activity. Recently, antibodies directed against carbamylated antigens (anti-CarP antibodies) were identified. A total of 120 RA patients were tested for anti-CCP antibodies using different methods and for anti-CarP antibodies using carbamylated fetal calf serum according to the method described by Shi et al. Additionally, ACPA fine specificities (to three citrullinated peptides) were measured. Disease activity was assessed at baseline using the disease activity score 28 (DAS28) in 80 patients. For 40 RA patients, joint erosion score (JES) was established. The median JES was 14.1 with a standard deviation of 11.5. Anti-CarP antibodies were correlated with joint erosion score (ρ = 0.34, 95 % CI 0.03–0.59; p = 0.0332). No correlation between ACPA and joint erosion score was observed. No individual marker correlated with DAS28. When one ACPA peptide was combined with anti-CarP antibodies in a score (ACPA peptide 1 divided by anti-CarP), a statistically relevant correlation was found (p = 0.0264). In this small cohort, the presence of anti-CarP antibodies, but not ACPA correlate with joint erosion score. Anti-CarP antibodies combined with ACPA fine specificities correlated with DAS28. Therefore, anti-CarP antibodies might represent a promising marker to predict joint damage and disease activity in RA patients.
| Original language | English |
|---|---|
| Pages (from-to) | 24-30 |
| Number of pages | 7 |
| Journal | Immunologic Research |
| Volume | 61 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2014 |
Fingerprint
Keywords
- Autoantibodies
- CarP
- CCP
- Citrullinated cyclic peptide
- Homocitrulline
- RA
- Rheumatoid arthritis
ASJC Scopus subject areas
- Immunology
- Medicine(all)
Cite this
Anti-CarP antibodies as promising marker to measure joint damage and disease activity in patients with rheumatoid arthritis. / Yee, Alvin; Webb, Tyler; Seaman, Andrea; Infantino, Maria; Meacci, Francesca; Manfredi, Mariangela; Benucci, Maurizio; Lakos, Gabriella; Favalli, Ennio; Shioppo, Tommaso; Meroni, Pier Luigi; Mahler, Michael.
In: Immunologic Research, Vol. 61, No. 1-2, 2014, p. 24-30.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-CarP antibodies as promising marker to measure joint damage and disease activity in patients with rheumatoid arthritis
AU - Yee, Alvin
AU - Webb, Tyler
AU - Seaman, Andrea
AU - Infantino, Maria
AU - Meacci, Francesca
AU - Manfredi, Mariangela
AU - Benucci, Maurizio
AU - Lakos, Gabriella
AU - Favalli, Ennio
AU - Shioppo, Tommaso
AU - Meroni, Pier Luigi
AU - Mahler, Michael
PY - 2014
Y1 - 2014
N2 - Anti-citrullinated protein antibodies (ACPA) are important serological markers in the diagnosis of rheumatoid arthritis (RA) and are part of the recent disease classification criteria. However, there is a strong need for reliable markers for measuring and predicting joint damage and disease activity. Recently, antibodies directed against carbamylated antigens (anti-CarP antibodies) were identified. A total of 120 RA patients were tested for anti-CCP antibodies using different methods and for anti-CarP antibodies using carbamylated fetal calf serum according to the method described by Shi et al. Additionally, ACPA fine specificities (to three citrullinated peptides) were measured. Disease activity was assessed at baseline using the disease activity score 28 (DAS28) in 80 patients. For 40 RA patients, joint erosion score (JES) was established. The median JES was 14.1 with a standard deviation of 11.5. Anti-CarP antibodies were correlated with joint erosion score (ρ = 0.34, 95 % CI 0.03–0.59; p = 0.0332). No correlation between ACPA and joint erosion score was observed. No individual marker correlated with DAS28. When one ACPA peptide was combined with anti-CarP antibodies in a score (ACPA peptide 1 divided by anti-CarP), a statistically relevant correlation was found (p = 0.0264). In this small cohort, the presence of anti-CarP antibodies, but not ACPA correlate with joint erosion score. Anti-CarP antibodies combined with ACPA fine specificities correlated with DAS28. Therefore, anti-CarP antibodies might represent a promising marker to predict joint damage and disease activity in RA patients.
AB - Anti-citrullinated protein antibodies (ACPA) are important serological markers in the diagnosis of rheumatoid arthritis (RA) and are part of the recent disease classification criteria. However, there is a strong need for reliable markers for measuring and predicting joint damage and disease activity. Recently, antibodies directed against carbamylated antigens (anti-CarP antibodies) were identified. A total of 120 RA patients were tested for anti-CCP antibodies using different methods and for anti-CarP antibodies using carbamylated fetal calf serum according to the method described by Shi et al. Additionally, ACPA fine specificities (to three citrullinated peptides) were measured. Disease activity was assessed at baseline using the disease activity score 28 (DAS28) in 80 patients. For 40 RA patients, joint erosion score (JES) was established. The median JES was 14.1 with a standard deviation of 11.5. Anti-CarP antibodies were correlated with joint erosion score (ρ = 0.34, 95 % CI 0.03–0.59; p = 0.0332). No correlation between ACPA and joint erosion score was observed. No individual marker correlated with DAS28. When one ACPA peptide was combined with anti-CarP antibodies in a score (ACPA peptide 1 divided by anti-CarP), a statistically relevant correlation was found (p = 0.0264). In this small cohort, the presence of anti-CarP antibodies, but not ACPA correlate with joint erosion score. Anti-CarP antibodies combined with ACPA fine specificities correlated with DAS28. Therefore, anti-CarP antibodies might represent a promising marker to predict joint damage and disease activity in RA patients.
KW - Autoantibodies
KW - CarP
KW - CCP
KW - Citrullinated cyclic peptide
KW - Homocitrulline
KW - RA
KW - Rheumatoid arthritis
UR - http://www.scopus.com/inward/record.url?scp=84925518142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84925518142&partnerID=8YFLogxK
U2 - 10.1007/s12026-014-8560-x
DO - 10.1007/s12026-014-8560-x
M3 - Article
C2 - 25391608
AN - SCOPUS:84925518142
VL - 61
SP - 24
EP - 30
JO - Immunologic Research
JF - Immunologic Research
SN - 0257-277X
IS - 1-2
ER -