Anti-CD30 immunotoxins with native and recombinant dianthin 30

Andrea Bolognesi, Pier Luigi Tazzari, Guiseppe Legname, Fabiola Olivieri, Daniela Modena, Roberto Conte, Fiorenzo Stirpe

Research output: Contribution to journalArticle

Abstract

Immunotoxins were prepared with a Ber-H2 (anti-CD30) monoclonal antibody and native or recombinant dianthin 30, a ribosome-inactivating protein from Dianthus caryophyllus (carnation). Both immunotoxins selectively inhibited protein synthesis by CD30+ cell lines D430B (lymphoblastoid, infected with Epstein-Barr virus). L428 and L540 (both from Hodgkin's lymphoma). IC50 values (concentrations, as dianthin, causing 50% inhibition) ranged from 324 pM to 479 pM (immunotoxin with native dianthin 30) or from 45 pM to 182 pM (immunotoxin with recombinant dianthin 30). The effect of either immunotoxin on protein synthesis by the CD30- cell line K562 (from a chronic myeloid leukaemia) was not different from that of free dianthin (IC50 higher than nM).

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalCancer Immunology, Immunotherapy
Volume40
Issue number2
DOIs
Publication statusPublished - Mar 1995

Keywords

  • Dianthin
  • Hodgkin's lymphoma
  • Immunotoxin
  • Ribosome-inactivating protein

ASJC Scopus subject areas

  • Oncology
  • Immunology
  • Cancer Research

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  • Cite this

    Bolognesi, A., Tazzari, P. L., Legname, G., Olivieri, F., Modena, D., Conte, R., & Stirpe, F. (1995). Anti-CD30 immunotoxins with native and recombinant dianthin 30. Cancer Immunology, Immunotherapy, 40(2), 109-114. https://doi.org/10.1007/BF01520292