Anti-cell antibodies in exposed seronegative individuals with HIV type 1-neutralizing activity

Lucia Lopalco, Claudia Pastori, Antonio Cosma, Samuele E. Burastero, Brunella Capiluppi, Enzo Boeri, Alberto Beretta, Adriano Lazzarin, Antonio G. Siccardi

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Abstract

Despite repeated exposures to HIV-1, some individuals remain seronegative. This study reports that sera from a fraction of exposed seronegative (ESN) subjects showed HIV-neutralizing activity; 5 of 17 ESN sera and none of 17 controls neutralized two different HIV-1 primary isolates (range of neutralizing titers: 1/20 to 1/60). The neutralizing activity was associated with the IgG fraction of 4 of 4 neutralizing ESN sera. Moreover, in 11 of 17 and 9 of 17 ESN sera (but none of the control sera) we found antibodies against HLA class I and CD4, respectively. One of the ESN sera (EU22) neutralized efficiently the primary virus derived from the seropositive partner and showed a good broadly cross-reactive neutralization. Immunoadsorption of two IgG fractions from EU19 and EU22 on peripheral blood mononuclear cells (PBMC) removed virus-neutralizing antibodies. The correlations between the ESN status and neutralizing activity (p <0.05), anti-HLA antibodies (p <0.0002), and anti-CD4 antibodies (p <0.001) were statistically significant. However, there was no statistically significant correlation between neutralizing activity and either anti-HLA or anti-CD4 antibodies. It can therefore be said that exposure to HIV-1 without seroconversion is, in some individuals, associated with HIV-neutralizing antibodies (not directed against viral antigens) and/or with anti-cell autoantibodies, which are possibly specific for cellular antigens involved in the infection/entry process.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume16
Issue number2
DOIs
Publication statusPublished - 2000

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HIV-1
Anti-Idiotypic Antibodies
Serum
Neutralizing Antibodies
Immunoglobulin G
Viruses
HIV Antibodies
Viral Antigens
Autoantibodies
Blood Cells
HIV
Antigens
Antibodies
Infection

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Anti-cell antibodies in exposed seronegative individuals with HIV type 1-neutralizing activity. / Lopalco, Lucia; Pastori, Claudia; Cosma, Antonio; Burastero, Samuele E.; Capiluppi, Brunella; Boeri, Enzo; Beretta, Alberto; Lazzarin, Adriano; Siccardi, Antonio G.

In: AIDS Research and Human Retroviruses, Vol. 16, No. 2, 2000, p. 109-115.

Research output: Contribution to journalArticle

Lopalco, Lucia ; Pastori, Claudia ; Cosma, Antonio ; Burastero, Samuele E. ; Capiluppi, Brunella ; Boeri, Enzo ; Beretta, Alberto ; Lazzarin, Adriano ; Siccardi, Antonio G. / Anti-cell antibodies in exposed seronegative individuals with HIV type 1-neutralizing activity. In: AIDS Research and Human Retroviruses. 2000 ; Vol. 16, No. 2. pp. 109-115.
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