Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon

L. Riente, B. Marchini, M. P. Dolcher, A. Puccetti, S. Bombardieri, P. Migliorini

Research output: Contribution to journalArticle

Abstract

The frequency and specificity of antibodies to native and denatured collagens were evaluated in systemic sclerosis (SSc) and in primary Raynaud's phenomenon (PRP) by direct and competitive ELISA. Antibodies reactive with denatured collagen type I (CI) were found in 43% of the SSc sera, and anti-CIV and anti-CV in 31%. In PRP, anti-CI, anti-CIV and anti-CV antibodies were detected in 8% of patient sera. Anti-CI, anti-CIV and anti-CV antibodies reacted with determinants expressed on the native as well as on the denatured molecule. Anti-CI and anti-CIV were cross-reactive; a reactivity with CII and a lower one with CV were detected. Anti-CV antibodies also reacted with CI and CII and, in a smaller proportion of cases, with CIV. Anti-collagen antibodies, affinity-purified from blotted collagen IV and V and cyanogen bromide (CBr)-digested CI, displayed the cross-reactivities shown by inhibition studies on sera. Moreover, antibodies eluted from a CBr fragment of CI reacted with the other CBr fragments as well. These data show that one-third of SSc sera contain antibodies that react with epitopes expressed on native as well as on heat-denatured CI, CII, CIV and CV, and therefore have the potential to bind collagens in vivo.

Original languageEnglish
Pages (from-to)354-359
Number of pages6
JournalClinical and Experimental Immunology
Volume102
Issue number2
Publication statusPublished - 1995

Fingerprint

Raynaud Disease
Systemic Scleroderma
Collagen Type I
Anti-Idiotypic Antibodies
Collagen
Cyanogen Bromide
Serum
Antibodies
Antibody Affinity
Antibody Specificity
Epitopes
Hot Temperature
Enzyme-Linked Immunosorbent Assay

Keywords

  • autoantibodies
  • collagen
  • Raynaud's phenomenon
  • systemic sclerosis

ASJC Scopus subject areas

  • Immunology

Cite this

Riente, L., Marchini, B., Dolcher, M. P., Puccetti, A., Bombardieri, S., & Migliorini, P. (1995). Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon. Clinical and Experimental Immunology, 102(2), 354-359.

Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon. / Riente, L.; Marchini, B.; Dolcher, M. P.; Puccetti, A.; Bombardieri, S.; Migliorini, P.

In: Clinical and Experimental Immunology, Vol. 102, No. 2, 1995, p. 354-359.

Research output: Contribution to journalArticle

Riente, L, Marchini, B, Dolcher, MP, Puccetti, A, Bombardieri, S & Migliorini, P 1995, 'Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon', Clinical and Experimental Immunology, vol. 102, no. 2, pp. 354-359.
Riente L, Marchini B, Dolcher MP, Puccetti A, Bombardieri S, Migliorini P. Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon. Clinical and Experimental Immunology. 1995;102(2):354-359.
Riente, L. ; Marchini, B. ; Dolcher, M. P. ; Puccetti, A. ; Bombardieri, S. ; Migliorini, P. / Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon. In: Clinical and Experimental Immunology. 1995 ; Vol. 102, No. 2. pp. 354-359.
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