Anti D dimer monoclonal antibodies: A possible scintigraphic agent for immunodetection of thrombi

F. Scopinaro, M. Di Loreto, M. Banci, O. Schillaci, L. Dimacio, R. De Santis, A. M. Anastasi, W. Clancarella, A. Centi Colella, A. Mele

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this work was to produce a MoAb able to react with clots but not with fibrinogen. Monoclonal antibodies directed towards DD dimers, against specific products of plasmic digestion of cross-linked fibrin, were obtained. One of these antibodies, F 60/43/8, showed a 1.79 × 1091 mol-1binding constant in spite of the presence of fibrinogen at a 4000 times greater concentration than the cross-linked fibrin. In vitro studies with 125I-F(ab')2of F 60/43/8 showed that 34-80% of the radioactivity can be found in human clots, in the presence of physiologic concentrations of fibrinogen, and that 96-h washing does not remove the labelled F(ab')2from the clot.131l-F(ab')2was injected into rabbits in which a clot had formed in an artery (six rabbits) or in a vein (six rabbits) of the left ear. Scintigraphic images of the clot were always obtained. In conclusion, the results of this work suggest that F 60/43/8 may be used as a specific antibody for the radioimmunodetection of thrombi.

Original languageEnglish
Pages (from-to)723-729
Number of pages7
JournalNuclear Medicine Communications
Volume13
Issue number10
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

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