Abstract
IgM M-proteins in some motor neuron disease (MND) patients bind immunologically to shared determinants on gangliosides GM1 and GD1b. Since patients with these M-proteins have improved with immunotherapy the antibodies may be important in the pathogenesis of MND. To study how the M-proteins might damage motor neurons, we established co-cultures of human neurons from spinal cord explants and human myotubes. Antibodies from patient but not control serum bound to the cultured neurons. Neurons in co-cultures degenerated after incubation with patient but not control serum. These results demonstrate that anti-GM1 antibodies can bind to and destroy spinal cord neurons that are cultured with muscle. Nerve-muscle co-cultures can serve as a system to examine effects of anti-GM1/GD1b M-proteins on motor neurons.
| Original language | English |
|---|---|
| Pages (from-to) | 38-45 |
| Number of pages | 8 |
| Journal | Journal of the Neurological Sciences |
| Volume | 120 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Dec 1 1993 |
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Keywords
- Anti-GM M-proteins
- Motor neurons
- Tissue culture
ASJC Scopus subject areas
- Ageing
- Clinical Neurology
- Surgery
- Neuroscience(all)
- Developmental Neuroscience
- Neurology
Cite this
Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle. / Heiman-Patterson, Terry; Krupa, Thomas; Thompson, Preston; Nobile-Orazio, Eduardo; Tahmoush, Albert J.; Shy, Michael E.
In: Journal of the Neurological Sciences, Vol. 120, No. 1, 01.12.1993, p. 38-45.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle
AU - Heiman-Patterson, Terry
AU - Krupa, Thomas
AU - Thompson, Preston
AU - Nobile-Orazio, Eduardo
AU - Tahmoush, Albert J.
AU - Shy, Michael E.
PY - 1993/12/1
Y1 - 1993/12/1
N2 - IgM M-proteins in some motor neuron disease (MND) patients bind immunologically to shared determinants on gangliosides GM1 and GD1b. Since patients with these M-proteins have improved with immunotherapy the antibodies may be important in the pathogenesis of MND. To study how the M-proteins might damage motor neurons, we established co-cultures of human neurons from spinal cord explants and human myotubes. Antibodies from patient but not control serum bound to the cultured neurons. Neurons in co-cultures degenerated after incubation with patient but not control serum. These results demonstrate that anti-GM1 antibodies can bind to and destroy spinal cord neurons that are cultured with muscle. Nerve-muscle co-cultures can serve as a system to examine effects of anti-GM1/GD1b M-proteins on motor neurons.
AB - IgM M-proteins in some motor neuron disease (MND) patients bind immunologically to shared determinants on gangliosides GM1 and GD1b. Since patients with these M-proteins have improved with immunotherapy the antibodies may be important in the pathogenesis of MND. To study how the M-proteins might damage motor neurons, we established co-cultures of human neurons from spinal cord explants and human myotubes. Antibodies from patient but not control serum bound to the cultured neurons. Neurons in co-cultures degenerated after incubation with patient but not control serum. These results demonstrate that anti-GM1 antibodies can bind to and destroy spinal cord neurons that are cultured with muscle. Nerve-muscle co-cultures can serve as a system to examine effects of anti-GM1/GD1b M-proteins on motor neurons.
KW - Anti-GM M-proteins
KW - Motor neurons
KW - Tissue culture
UR - http://www.scopus.com/inward/record.url?scp=0027786943&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027786943&partnerID=8YFLogxK
U2 - 10.1016/0022-510X(93)90022-Q
DO - 10.1016/0022-510X(93)90022-Q
M3 - Article
C2 - 8289078
AN - SCOPUS:0027786943
VL - 120
SP - 38
EP - 45
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
SN - 0022-510X
IS - 1
ER -