Anti HL A inhibitors in supernatants of phytohaemagglutinin activated human lymphocyte cultures

C. Rugarli, R. Scorza, M. Venegoni, M. G. Sabbadini, C. Zanussi

Research output: Contribution to journalArticlepeer-review

Abstract

Human peripheral blood lymphocytes were cultured in vitro for 24 hr in the presence of phytohemagglutinin (PHA) and thereafter with fresh medium without mitogen for a 72 hr period. Supernatant media at the end of the cultures were harvested, filtered through Millipore filters (0.22 μm pore size), and employed in the lymphocytotoxicity test as an inhibiting material on anti HL A antibodies. Inhibition patterns, although inconstant, were specific and consistent with the hypothesis that soluble HL A antigens were released by PHA stimulated lymphocytes. Titration of such an inhibiting activity in the term of 'whole cell equivalents' showed that it was nearly 100 fold greater than the number of cultured lymphocytes producing it. The results and their serological implications are discussed, including the nature of the inhibiting material.

Original languageEnglish
Pages (from-to)417-428
Number of pages12
JournalBollettino dell'Istituto Sieroterapico Milanese
Volume52
Issue number5-6
Publication statusPublished - 1973

ASJC Scopus subject areas

  • Immunology

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