Anti-human thyroglobulin autoantibodies assayed by an enzyme-linked immunosorbent assay

Marcello Bagnasco, Silvano Ferrini, Annalisa Merli, Walter Dirienzo, Giorgio Ciprandi, Giovanni Melioli

Research output: Contribution to journalArticle

Abstract

A rapid and simple enzyme-linked immunosorbent assay (ELISA) for anti-thyroglobulin IgG antibodies is described. The method is based on a 'sandwich' using purified human thyroglobulin adsorbed to polystyrene microplates, human serum and anti-human IgG antiserum conjugated to alkaline phosphatase. The sensitivity of the method is about 8 ng/ml. as evaluated with a purified anti-thyroglobulin antibody preparation. High concentrations of antibodies were observed, as expected, in autoimmune thyroid disease; however, the majority of normal subjects have detectable, although very low, antibody levels. We conclude the method is suitable for current clinical use.

Original languageEnglish
Pages (from-to)247-254
Number of pages8
JournalLa Ricerca in Clinica e in Laboratorio
Volume13
Issue number2
DOIs
Publication statusPublished - Apr 1983

Keywords

  • Anti-thyroglobulin autoantibodies
  • Enzyme-linked immunosorbent assay (ELISA)
  • Graves disease
  • Hashimoto's thyroiditis
  • Thyroglobulin

ASJC Scopus subject areas

  • Clinical Biochemistry

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  • Cite this

    Bagnasco, M., Ferrini, S., Merli, A., Dirienzo, W., Ciprandi, G., & Melioli, G. (1983). Anti-human thyroglobulin autoantibodies assayed by an enzyme-linked immunosorbent assay. La Ricerca in Clinica e in Laboratorio, 13(2), 247-254. https://doi.org/10.1007/BF02904838