Abstract
Type II keratins from cashmere are used as antigens to produce species-specific monoclonal antibodies for fiber identification purposes. Balb/c mice are immunized with the keratins purified by two-dimensional preparative gel electrophoresis. Hybridoma clones showing high immunological responses are subcloned by limiting dilution and then expanded to obtain antibodies in large amounts. When the antibodies are screened by two-dimensional immunoblotting for immunoreactivity with keratins isolated from cashmere and wool, several differences are detected, both quantitative and qualitative. Although further work is needed to develop routine analytical protocols, the preliminary results reported in this paper appear to be extremely promising, and our approach could contribute to solving the problem of the objective identification of cashmere and other speciality animal fibers.
| Original language | English |
|---|---|
| Pages (from-to) | 458-464 |
| Number of pages | 7 |
| Journal | Textile Research Journal |
| Volume | 74 |
| Issue number | 5 |
| Publication status | Published - May 2004 |
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ASJC Scopus subject areas
- Polymers and Plastics
- Chemical Engineering (miscellaneous)
Cite this
Anti-keratin monoclonal antibodies for identifying animal hair fibers. / Paluzzi, Silvio; Mormino, Michele; Vineis, Claudia; Tonin, Claudio; Patrone, Eligio; Barboro, Paola; Balbi, Cecilia.
In: Textile Research Journal, Vol. 74, No. 5, 05.2004, p. 458-464.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-keratin monoclonal antibodies for identifying animal hair fibers
AU - Paluzzi, Silvio
AU - Mormino, Michele
AU - Vineis, Claudia
AU - Tonin, Claudio
AU - Patrone, Eligio
AU - Barboro, Paola
AU - Balbi, Cecilia
PY - 2004/5
Y1 - 2004/5
N2 - Type II keratins from cashmere are used as antigens to produce species-specific monoclonal antibodies for fiber identification purposes. Balb/c mice are immunized with the keratins purified by two-dimensional preparative gel electrophoresis. Hybridoma clones showing high immunological responses are subcloned by limiting dilution and then expanded to obtain antibodies in large amounts. When the antibodies are screened by two-dimensional immunoblotting for immunoreactivity with keratins isolated from cashmere and wool, several differences are detected, both quantitative and qualitative. Although further work is needed to develop routine analytical protocols, the preliminary results reported in this paper appear to be extremely promising, and our approach could contribute to solving the problem of the objective identification of cashmere and other speciality animal fibers.
AB - Type II keratins from cashmere are used as antigens to produce species-specific monoclonal antibodies for fiber identification purposes. Balb/c mice are immunized with the keratins purified by two-dimensional preparative gel electrophoresis. Hybridoma clones showing high immunological responses are subcloned by limiting dilution and then expanded to obtain antibodies in large amounts. When the antibodies are screened by two-dimensional immunoblotting for immunoreactivity with keratins isolated from cashmere and wool, several differences are detected, both quantitative and qualitative. Although further work is needed to develop routine analytical protocols, the preliminary results reported in this paper appear to be extremely promising, and our approach could contribute to solving the problem of the objective identification of cashmere and other speciality animal fibers.
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UR - http://www.scopus.com/inward/citedby.url?scp=2542495026&partnerID=8YFLogxK
M3 - Article
VL - 74
SP - 458
EP - 464
JO - Textile Reseach Journal
JF - Textile Reseach Journal
SN - 0040-5175
IS - 5
ER -