Anti-lymphocyte globulin stimulates normal human T cells to proliferate and to release lymphokines in vitro. A study at the clonal level

G. C. Barbano, A. Schenone, S. Roncella, R. Ghio, A. Corcione, P. G. Mori, V. Pistoia, M. Ferrarini

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21 Citations (Scopus)

Abstract

Human peripheral blood mononuclear cells (PBMC) were stimulated in vitro with anti-lymphocyte globulin (ALG), and the phenotypic and functional properties of the blasts obtained were investigated. When stained with monoclonal antibodies (MoAbs), all of the blasts were identified as T cells that expressed predominantly the CD4 phenotype (70% of the cells). The remaining blasts were CD8+. These findings demonstrate that ALG stimulates both helper-inducer and cytotoxic-suppressor cells at random since the Cd4 to Cd8 ratio in the stimulated blasts was the same as in resting PBMC. This ratio is different from that observed in short-term cultures of T cells stimulated with phytohemagglutinin (PHA) under the same conditions (CD4 to CD8 ratio

Original languageEnglish
Pages (from-to)956-963
Number of pages8
JournalBlood
Volume72
Issue number3
Publication statusPublished - 1988

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T-cells
Lymphocytes
Lymphokines
Globulins
Blood Cells
Blood
T-Lymphocytes
CD4-CD8 Ratio
Phytohemagglutinins
Cell culture
Monoclonal Antibodies
Phenotype
In Vitro Techniques

ASJC Scopus subject areas

  • Hematology

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Anti-lymphocyte globulin stimulates normal human T cells to proliferate and to release lymphokines in vitro. A study at the clonal level. / Barbano, G. C.; Schenone, A.; Roncella, S.; Ghio, R.; Corcione, A.; Mori, P. G.; Pistoia, V.; Ferrarini, M.

In: Blood, Vol. 72, No. 3, 1988, p. 956-963.

Research output: Contribution to journalArticle

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AU - Schenone, A.

AU - Roncella, S.

AU - Ghio, R.

AU - Corcione, A.

AU - Mori, P. G.

AU - Pistoia, V.

AU - Ferrarini, M.

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