Anti-mitochondrial autoantibodies in primary biliary cirrhosis by radioimmunoassay and enzyme immunoassay

M. Plebani, R. Bertorelle, L. Sciacovelli, M. Chiaromonte, A. Burlina

Research output: Contribution to journalArticle

Abstract

We have evaluated a radioimmunoassay and an enzyme immunoassay for the determination of a specific subtype of antimitochondrial antibodies in patients with primary biliary cirrhosis. Both methods show a sufficient precision. (CV% from 5.0 to 11.2 for EIA and from 4.1 to 8.1 for RIA) and a wide range of measurement, and they do not seem to be affected by endogenous constituents or by rheumatoid factor. A good correlation between the two techniques was found (r=0.957; RIA=2.366EIA-83.893). Finally, the sensitivity, specificity and accuracy of both methods were calculated in patients with primary biliary cirrhosis and chronic active hepatitis and in controls. The sensitivity of the assays (81 for EIA, 84 for RIA) is not sufficient for them to replace commonly used indirect immunofluorescence techniques.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalClinical Chemistry and Enzymology Communications
Volume1
Issue number1
Publication statusPublished - 1988

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Biliary Liver Cirrhosis
Rheumatoid Factor
Immunoenzyme Techniques
Autoantibodies
Radioimmunoassay
Assays
Antibodies
Enzymes
Chronic Hepatitis
Indirect Fluorescent Antibody Technique
Sensitivity and Specificity

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Anti-mitochondrial autoantibodies in primary biliary cirrhosis by radioimmunoassay and enzyme immunoassay. / Plebani, M.; Bertorelle, R.; Sciacovelli, L.; Chiaromonte, M.; Burlina, A.

In: Clinical Chemistry and Enzymology Communications, Vol. 1, No. 1, 1988, p. 63-69.

Research output: Contribution to journalArticle

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