Anti-peptide antibodies that recognize conformational differences of HLA class I intracytoplasmic domains

Alberto Chersi, Rossella Galati, Takeshi Ogino, Richard H. Butler, Nobuyuki Tanigaki

Research output: Contribution to journalArticlepeer-review


Rabbit antibodies were raised against both long and short peptides derived from exon 7 sequences of human leukocyte antigen (HLA) class I α chains; anti-A/B against a 13-mer shared by most HLA-A α and HLA-B α chains, anti-C against a 15-mer characteristic of HLA-C α chains, anti-ACT against a 6-mer specific to HLA-A α chains, and anti-CCT against a 5-mer specific to HLA-C α chains. Binding activity of the antibodies was determined with peptides by enzyme-linked immunoabsorbent assay (ELISA) and with HLA class I transfectants and the parental cells by FACS analysis. Anti-A/B and anti-C were to a greater or lesser extent crossreactive with the long and short peptides, whereas anti-ACT and anti-CCT were specific to the corresponding short peptides. No binding was seen for anti-ACT and anti-CCT with HLA class I transfectants, C1R-A2, C1R-B7, and 221-Cw1 and the parental cells, C1R (Cw4, E) and 721.221 (E, F). Anti-A/B and anti-C were substantially protein-reactive and the binding order was C1R-B7 > C1R-A2, 721.221 > C1R, 221-Cwl for anti-A/B, and C1R-B7 > 721.221 > C1R, 221-Cwl, C1R-A2 for anti-C. Thus, anti-A/B and anti-C bound better to HLA-B and HLA-E rather than to HLA-A and HLA-C. Computer modeling of the three- dimensional structure of the intracytoplasmic domains demonstrated that this may be due to structural differences despite the sequence similarities.

Original languageEnglish
Pages (from-to)731-741
Number of pages11
JournalHuman Immunology
Issue number9
Publication statusPublished - Sep 2002


  • Anti-peptide antibodies
  • Computer modeling
  • HLA class I intracytoplasmic domains

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy


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