Anti-Th/To are common antinucleolar autoantibodies in Italian patients with scleroderma

Angela Ceribelli, Ilaria Cavazzana, Franco Franceschini, Paolo Airò, Angela Tincani, Roberto Cattaneo, Brad A. Pauley, Edward K L Chan, Minoru Satoh

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Objective. Patients with scleroderma (systemic sclerosis; SSc) can be classified into subsets based on autoantibody profile and clinical features. Specificities such as anti-Th/To and anti-fibrillarin (U3RNP) are detectable mainly by immunoprecipitation (IP), which is not widely used in clinical laboratories. We examined the autoantibody profiles and clinical manifestations in a cohort of Italian patients with SSc, focusing on anti-Th/To and anticentromere (ACA) antibodies, associated with limited cutaneous SSc (lcSSc). Methods. Sera from 216 consecutive patients with SSc were tested for ACA (by indirect immunofluorescence), antitopoisomerase I (topo I; by counterimmunoelectrophoresis), and anti-RNA polymerase III (RNAPIII; by ELISA). Forty-one sera negative for these specificities were tested by IP analysis of proteins (35S-methionine labeled K562 cell extract) and RNA (silver staining). Results. Among 216 SSc patients analyzed, anti-topo I, ACA, and anti-RNAPIII were detected in 38% (81/216), 31% (67/216) and 7% (15/216), respectively. Among 41 sera negative for ACA, anti-topo I, and anti-RNAPIII and which were tested by IP, 14 were nucleolar stain-positive. Eight out of 14 (57%) showed anti-Th/To reactivity, but no anti-U3RNP was found. In comparison with ACA-positive patients, anti-Th/To-positive patients were younger (p = 0.0046) and more commonly were male (p = 0.0006). All 8 anti-Th/To-positive and all but one ACA-positive patients had lcSSc. Interstitial lung disease (ILD) and pericarditis were more frequent in anti-Th/To-positive patients. Conclusion. Anti-Th/To are common in antinucleolar antibody-positive Italian patients with SSc. Anti-Th/To and ACA patients had lcSSc, with excellent prognosis. The anti-Th/To group had frequent pericarditis and ILD, although impairment of pulmonary function appeared mild. The Journal of Rheumatology

Original languageEnglish
Pages (from-to)2071-2075
Number of pages5
JournalJournal of Rheumatology
Volume37
Issue number10
DOIs
Publication statusPublished - Oct 2010

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Autoantibodies
Immunoprecipitation
Pericarditis
Interstitial Lung Diseases
Skin
Serum
Counterimmunoelectrophoresis
RNA Polymerase III
Silver Staining
K562 Cells
Systemic Scleroderma
Rheumatology
Indirect Fluorescent Antibody Technique
Cell Extracts
Methionine
Coloring Agents
Enzyme-Linked Immunosorbent Assay
RNA
Lung
Antibodies

Keywords

  • Anti-Th/To antibodies
  • Anticentromere antibodies
  • Antinucleolar antibodies
  • RNA-immunoprecipitation
  • Systemic sclerosis

ASJC Scopus subject areas

  • Rheumatology
  • Immunology
  • Immunology and Allergy

Cite this

Ceribelli, A., Cavazzana, I., Franceschini, F., Airò, P., Tincani, A., Cattaneo, R., ... Satoh, M. (2010). Anti-Th/To are common antinucleolar autoantibodies in Italian patients with scleroderma. Journal of Rheumatology, 37(10), 2071-2075. https://doi.org/10.3899/jrheum.100316

Anti-Th/To are common antinucleolar autoantibodies in Italian patients with scleroderma. / Ceribelli, Angela; Cavazzana, Ilaria; Franceschini, Franco; Airò, Paolo; Tincani, Angela; Cattaneo, Roberto; Pauley, Brad A.; Chan, Edward K L; Satoh, Minoru.

In: Journal of Rheumatology, Vol. 37, No. 10, 10.2010, p. 2071-2075.

Research output: Contribution to journalArticle

Ceribelli, A, Cavazzana, I, Franceschini, F, Airò, P, Tincani, A, Cattaneo, R, Pauley, BA, Chan, EKL & Satoh, M 2010, 'Anti-Th/To are common antinucleolar autoantibodies in Italian patients with scleroderma', Journal of Rheumatology, vol. 37, no. 10, pp. 2071-2075. https://doi.org/10.3899/jrheum.100316
Ceribelli, Angela ; Cavazzana, Ilaria ; Franceschini, Franco ; Airò, Paolo ; Tincani, Angela ; Cattaneo, Roberto ; Pauley, Brad A. ; Chan, Edward K L ; Satoh, Minoru. / Anti-Th/To are common antinucleolar autoantibodies in Italian patients with scleroderma. In: Journal of Rheumatology. 2010 ; Vol. 37, No. 10. pp. 2071-2075.
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abstract = "Objective. Patients with scleroderma (systemic sclerosis; SSc) can be classified into subsets based on autoantibody profile and clinical features. Specificities such as anti-Th/To and anti-fibrillarin (U3RNP) are detectable mainly by immunoprecipitation (IP), which is not widely used in clinical laboratories. We examined the autoantibody profiles and clinical manifestations in a cohort of Italian patients with SSc, focusing on anti-Th/To and anticentromere (ACA) antibodies, associated with limited cutaneous SSc (lcSSc). Methods. Sera from 216 consecutive patients with SSc were tested for ACA (by indirect immunofluorescence), antitopoisomerase I (topo I; by counterimmunoelectrophoresis), and anti-RNA polymerase III (RNAPIII; by ELISA). Forty-one sera negative for these specificities were tested by IP analysis of proteins (35S-methionine labeled K562 cell extract) and RNA (silver staining). Results. Among 216 SSc patients analyzed, anti-topo I, ACA, and anti-RNAPIII were detected in 38{\%} (81/216), 31{\%} (67/216) and 7{\%} (15/216), respectively. Among 41 sera negative for ACA, anti-topo I, and anti-RNAPIII and which were tested by IP, 14 were nucleolar stain-positive. Eight out of 14 (57{\%}) showed anti-Th/To reactivity, but no anti-U3RNP was found. In comparison with ACA-positive patients, anti-Th/To-positive patients were younger (p = 0.0046) and more commonly were male (p = 0.0006). All 8 anti-Th/To-positive and all but one ACA-positive patients had lcSSc. Interstitial lung disease (ILD) and pericarditis were more frequent in anti-Th/To-positive patients. Conclusion. Anti-Th/To are common in antinucleolar antibody-positive Italian patients with SSc. Anti-Th/To and ACA patients had lcSSc, with excellent prognosis. The anti-Th/To group had frequent pericarditis and ILD, although impairment of pulmonary function appeared mild. The Journal of Rheumatology",
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AU - Ceribelli, Angela

AU - Cavazzana, Ilaria

AU - Franceschini, Franco

AU - Airò, Paolo

AU - Tincani, Angela

AU - Cattaneo, Roberto

AU - Pauley, Brad A.

AU - Chan, Edward K L

AU - Satoh, Minoru

PY - 2010/10

Y1 - 2010/10

N2 - Objective. Patients with scleroderma (systemic sclerosis; SSc) can be classified into subsets based on autoantibody profile and clinical features. Specificities such as anti-Th/To and anti-fibrillarin (U3RNP) are detectable mainly by immunoprecipitation (IP), which is not widely used in clinical laboratories. We examined the autoantibody profiles and clinical manifestations in a cohort of Italian patients with SSc, focusing on anti-Th/To and anticentromere (ACA) antibodies, associated with limited cutaneous SSc (lcSSc). Methods. Sera from 216 consecutive patients with SSc were tested for ACA (by indirect immunofluorescence), antitopoisomerase I (topo I; by counterimmunoelectrophoresis), and anti-RNA polymerase III (RNAPIII; by ELISA). Forty-one sera negative for these specificities were tested by IP analysis of proteins (35S-methionine labeled K562 cell extract) and RNA (silver staining). Results. Among 216 SSc patients analyzed, anti-topo I, ACA, and anti-RNAPIII were detected in 38% (81/216), 31% (67/216) and 7% (15/216), respectively. Among 41 sera negative for ACA, anti-topo I, and anti-RNAPIII and which were tested by IP, 14 were nucleolar stain-positive. Eight out of 14 (57%) showed anti-Th/To reactivity, but no anti-U3RNP was found. In comparison with ACA-positive patients, anti-Th/To-positive patients were younger (p = 0.0046) and more commonly were male (p = 0.0006). All 8 anti-Th/To-positive and all but one ACA-positive patients had lcSSc. Interstitial lung disease (ILD) and pericarditis were more frequent in anti-Th/To-positive patients. Conclusion. Anti-Th/To are common in antinucleolar antibody-positive Italian patients with SSc. Anti-Th/To and ACA patients had lcSSc, with excellent prognosis. The anti-Th/To group had frequent pericarditis and ILD, although impairment of pulmonary function appeared mild. The Journal of Rheumatology

AB - Objective. Patients with scleroderma (systemic sclerosis; SSc) can be classified into subsets based on autoantibody profile and clinical features. Specificities such as anti-Th/To and anti-fibrillarin (U3RNP) are detectable mainly by immunoprecipitation (IP), which is not widely used in clinical laboratories. We examined the autoantibody profiles and clinical manifestations in a cohort of Italian patients with SSc, focusing on anti-Th/To and anticentromere (ACA) antibodies, associated with limited cutaneous SSc (lcSSc). Methods. Sera from 216 consecutive patients with SSc were tested for ACA (by indirect immunofluorescence), antitopoisomerase I (topo I; by counterimmunoelectrophoresis), and anti-RNA polymerase III (RNAPIII; by ELISA). Forty-one sera negative for these specificities were tested by IP analysis of proteins (35S-methionine labeled K562 cell extract) and RNA (silver staining). Results. Among 216 SSc patients analyzed, anti-topo I, ACA, and anti-RNAPIII were detected in 38% (81/216), 31% (67/216) and 7% (15/216), respectively. Among 41 sera negative for ACA, anti-topo I, and anti-RNAPIII and which were tested by IP, 14 were nucleolar stain-positive. Eight out of 14 (57%) showed anti-Th/To reactivity, but no anti-U3RNP was found. In comparison with ACA-positive patients, anti-Th/To-positive patients were younger (p = 0.0046) and more commonly were male (p = 0.0006). All 8 anti-Th/To-positive and all but one ACA-positive patients had lcSSc. Interstitial lung disease (ILD) and pericarditis were more frequent in anti-Th/To-positive patients. Conclusion. Anti-Th/To are common in antinucleolar antibody-positive Italian patients with SSc. Anti-Th/To and ACA patients had lcSSc, with excellent prognosis. The anti-Th/To group had frequent pericarditis and ILD, although impairment of pulmonary function appeared mild. The Journal of Rheumatology

KW - Anti-Th/To antibodies

KW - Anticentromere antibodies

KW - Antinucleolar antibodies

KW - RNA-immunoprecipitation

KW - Systemic sclerosis

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