Anti-tumor immunity induced by murine melanoma cells transduced with the Mycobacterium tuberculosis gene encoding the 38-kDa antigen

L. Sfondrini, D. Morelli, S. Ménard, Jam Maier, M. Singh, C. Melani, N. Terrazzini, M. P. Colombo, M. I. Colnaghi, A. Balsari

Research output: Contribution to journalArticlepeer-review

Abstract

The Mycobacterium tuberculosis Ag38 gene, which encodes a highly immunogenic protein, was cloned into a retroviral vector in-frame with the leader and the trans-membrane portion of the nerve growth factor receptor, and transduced into murine melanoma cell line B16-B78. Significant protection was observed in mice immunized with the transduced melanoma cells and subcutaneously challenged with parental melanoma cells since only 20% of mice developed tumors. Necroscopy of mice immunized with the transduced melanoma cells revealed dramatic inhibition of experimental metastases induced by intravenous (i.v.) inoculation of parental melanomas cells. Moreover, vaccination with transduced cells significantly prolonged survival of mice challenged i.v. with parental melanoma cells. These data indicate that the presence of the mycobacterial 38-kDa protein greatly enhances immunological recognition of structures expressed by the parental melanoma cells. Comparison of Th1 and Th2 responses in mice immunised with parental melanoma cells versus mice receiving the transduced cells revealed a clear predominance of Th1 responses when the Ag38 protein was endogenously expressed. This transduction approach may represent a promising immunotherapeutic strategy for the treatment of cancer patients.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalGene Therapy
Volume5
Issue number2
Publication statusPublished - 1998

Keywords

  • Cancer vaccine
  • Mouse melanoma
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Genetics

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