Anti-V3 and anti-IgG antibodies of healthy individuals share complementarity structures

Radmila Metlas, D. Trajkovic, T. Srdic, V. Veljkovic, A. Colombatti

Research output: Contribution to journalArticle

Abstract

It was recently shown that antibodies reactive with a peptide from the tip of the HIV-1(NY5) gp120 V3 loop (V3 peptide) are present not only in sera of HIV-positive patients but also in sera of healthy HIV-negative individuals. In the present study, we show that V3 peptide reactive antibodies are predominantly IgM in sera of HIV negative individuals and that a fraction of the IgG anti-V3 antibodies exhibit features of autoantibodies. These antibodies were purified by chromatography on IgG-sepharose columns from sera as well as from purified IgG anti-V3 antibodies. A higher IgG anti- V3 reactivity was detected in autoantibody preparations from HIV-positive sera as compared with the reactivity of sera and purified antibodies from HIV-negative individuals. This was confirmed by solid phase binding of IgG anti-V3 antibodies both to V3 and to human IgG F(ab')2 antigens. The autoantibodies did not bind to peptides that share sequence similarity with V3 peptide indicating a high epitope specificity. The detection of antibodies against HIV epitopes in HIV-negative individuals may suggest that anti-V3 antibodies after HIV infection represent at least in part a secondary immune response.

Original languageEnglish
Pages (from-to)266-270
Number of pages5
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume21
Issue number4
Publication statusPublished - Aug 1 1999

Fingerprint

Anti-Idiotypic Antibodies
HIV
Immunoglobulin G
Serum
Autoantibodies
HIV Antibodies
Peptides
Antibodies
Epitopes
HIV Infections
Immunoglobulin M
anti-IgG
HIV-1
Chromatography
Antigens

Keywords

  • Anti-V3 antibodies
  • Autoantibodies
  • gp 120 V3 loop
  • HIV

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology

Cite this

Anti-V3 and anti-IgG antibodies of healthy individuals share complementarity structures. / Metlas, Radmila; Trajkovic, D.; Srdic, T.; Veljkovic, V.; Colombatti, A.

In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, Vol. 21, No. 4, 01.08.1999, p. 266-270.

Research output: Contribution to journalArticle

Metlas, Radmila ; Trajkovic, D. ; Srdic, T. ; Veljkovic, V. ; Colombatti, A. / Anti-V3 and anti-IgG antibodies of healthy individuals share complementarity structures. In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 1999 ; Vol. 21, No. 4. pp. 266-270.
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