Abstract
It was recently shown that antibodies reactive with a peptide from the tip of the HIV-1(NY5) gp120 V3 loop (V3 peptide) are present not only in sera of HIV-positive patients but also in sera of healthy HIV-negative individuals. In the present study, we show that V3 peptide reactive antibodies are predominantly IgM in sera of HIV negative individuals and that a fraction of the IgG anti-V3 antibodies exhibit features of autoantibodies. These antibodies were purified by chromatography on IgG-sepharose columns from sera as well as from purified IgG anti-V3 antibodies. A higher IgG anti- V3 reactivity was detected in autoantibody preparations from HIV-positive sera as compared with the reactivity of sera and purified antibodies from HIV-negative individuals. This was confirmed by solid phase binding of IgG anti-V3 antibodies both to V3 and to human IgG F(ab')2 antigens. The autoantibodies did not bind to peptides that share sequence similarity with V3 peptide indicating a high epitope specificity. The detection of antibodies against HIV epitopes in HIV-negative individuals may suggest that anti-V3 antibodies after HIV infection represent at least in part a secondary immune response.
| Original language | English |
|---|---|
| Pages (from-to) | 266-270 |
| Number of pages | 5 |
| Journal | Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology |
| Volume | 21 |
| Issue number | 4 |
| Publication status | Published - Aug 1 1999 |
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Keywords
- Anti-V3 antibodies
- Autoantibodies
- gp 120 V3 loop
- HIV
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Virology
Cite this
Anti-V3 and anti-IgG antibodies of healthy individuals share complementarity structures. / Metlas, Radmila; Trajkovic, D.; Srdic, T.; Veljkovic, V.; Colombatti, A.
In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, Vol. 21, No. 4, 01.08.1999, p. 266-270.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-V3 and anti-IgG antibodies of healthy individuals share complementarity structures
AU - Metlas, Radmila
AU - Trajkovic, D.
AU - Srdic, T.
AU - Veljkovic, V.
AU - Colombatti, A.
PY - 1999/8/1
Y1 - 1999/8/1
N2 - It was recently shown that antibodies reactive with a peptide from the tip of the HIV-1(NY5) gp120 V3 loop (V3 peptide) are present not only in sera of HIV-positive patients but also in sera of healthy HIV-negative individuals. In the present study, we show that V3 peptide reactive antibodies are predominantly IgM in sera of HIV negative individuals and that a fraction of the IgG anti-V3 antibodies exhibit features of autoantibodies. These antibodies were purified by chromatography on IgG-sepharose columns from sera as well as from purified IgG anti-V3 antibodies. A higher IgG anti- V3 reactivity was detected in autoantibody preparations from HIV-positive sera as compared with the reactivity of sera and purified antibodies from HIV-negative individuals. This was confirmed by solid phase binding of IgG anti-V3 antibodies both to V3 and to human IgG F(ab')2 antigens. The autoantibodies did not bind to peptides that share sequence similarity with V3 peptide indicating a high epitope specificity. The detection of antibodies against HIV epitopes in HIV-negative individuals may suggest that anti-V3 antibodies after HIV infection represent at least in part a secondary immune response.
AB - It was recently shown that antibodies reactive with a peptide from the tip of the HIV-1(NY5) gp120 V3 loop (V3 peptide) are present not only in sera of HIV-positive patients but also in sera of healthy HIV-negative individuals. In the present study, we show that V3 peptide reactive antibodies are predominantly IgM in sera of HIV negative individuals and that a fraction of the IgG anti-V3 antibodies exhibit features of autoantibodies. These antibodies were purified by chromatography on IgG-sepharose columns from sera as well as from purified IgG anti-V3 antibodies. A higher IgG anti- V3 reactivity was detected in autoantibody preparations from HIV-positive sera as compared with the reactivity of sera and purified antibodies from HIV-negative individuals. This was confirmed by solid phase binding of IgG anti-V3 antibodies both to V3 and to human IgG F(ab')2 antigens. The autoantibodies did not bind to peptides that share sequence similarity with V3 peptide indicating a high epitope specificity. The detection of antibodies against HIV epitopes in HIV-negative individuals may suggest that anti-V3 antibodies after HIV infection represent at least in part a secondary immune response.
KW - Anti-V3 antibodies
KW - Autoantibodies
KW - gp 120 V3 loop
KW - HIV
UR - http://www.scopus.com/inward/record.url?scp=0033179410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033179410&partnerID=8YFLogxK
M3 - Article
C2 - 10428103
AN - SCOPUS:0033179410
VL - 21
SP - 266
EP - 270
JO - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
JF - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
SN - 1077-9450
IS - 4
ER -