Anti-V3 and anti-IgG antibodies of healthy individuals share complementarity structures

Radmila Metlas, D. Trajkovic, T. Srdic, V. Veljkovic, A. Colombatti

Research output: Contribution to journalArticlepeer-review


It was recently shown that antibodies reactive with a peptide from the tip of the HIV-1(NY5) gp120 V3 loop (V3 peptide) are present not only in sera of HIV-positive patients but also in sera of healthy HIV-negative individuals. In the present study, we show that V3 peptide reactive antibodies are predominantly IgM in sera of HIV negative individuals and that a fraction of the IgG anti-V3 antibodies exhibit features of autoantibodies. These antibodies were purified by chromatography on IgG-sepharose columns from sera as well as from purified IgG anti-V3 antibodies. A higher IgG anti- V3 reactivity was detected in autoantibody preparations from HIV-positive sera as compared with the reactivity of sera and purified antibodies from HIV-negative individuals. This was confirmed by solid phase binding of IgG anti-V3 antibodies both to V3 and to human IgG F(ab')2 antigens. The autoantibodies did not bind to peptides that share sequence similarity with V3 peptide indicating a high epitope specificity. The detection of antibodies against HIV epitopes in HIV-negative individuals may suggest that anti-V3 antibodies after HIV infection represent at least in part a secondary immune response.

Original languageEnglish
Pages (from-to)266-270
Number of pages5
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Issue number4
Publication statusPublished - Aug 1 1999


  • Anti-V3 antibodies
  • Autoantibodies
  • gp 120 V3 loop
  • HIV

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology


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