Antiangiogenic activity of semisynthetic biotechnological heparins: Low-molecular-weight-sulfated Escherichia coli K5 polysaccharide derivatives as fibroblast growth factor antagonists

Marco Presta, Pasqua Oreste, Giorgio Zoppetti, Mirella Belleri, Elena Tanghetti, Daria Leali, Chiara Urbinati, Antonella Bugatti, Roberto Ronca, Stefania Nicoli, Emanuela Moroni, Helena Stabile, Maura Camozzi, German Andrés Hernandez, Stefania Mitola, Patrizia Dell'Era, Marco Rusnati, Domenico Ribatti

Research output: Contribution to journalArticle

Abstract

Objective - Low-molecular-weight heparin (LMWH) exerts antitumor activity in clinical trials. The K5 polysaccharide from Escherichia coli has the same structure as the heparin precursor. Chemical and enzymatic modifications of K5 polysaccharide lead to the production of biotechnological heparin-like compounds. We investigated the fibroblast growth factor-2 (FGF2) antagonist and antiangiogenic activity of a series of LMW N,O-sulfated K5 derivatives. Methods and Results - Surface plasmon resonance analysis showed that LMW-K5 derivatives bind FGF2, thus inhibiting its interaction with heparin immobilized to a BIAcore sensor chip. Interaction of FGF2 with tyrosine-kinase receptors (FGFRs), heparan sulfate proteoglycans (HSPGs), and αvβ3 integrin is required for biological response in endothelial cells. Similar to LMWH, LMW-K5 derivatives abrogate the formation of HSPG/FGF2/FGFR ternary complexes by preventing FGF2-mediated attachment of FGFR1-overexpressing cells to HSPG-bearing cells and inhibit FGF2-mediated endothelial cell proliferation. However, LMW-K5 derivatives, but not LMWH, also inhibit FGF2/α vβ3 integrin interaction and consequent FGF2-mediated endothelial cell sprouting in vitro and angiogenesis in vivo in the chick embryo chorioallantoic membrane. Conclusions - LMW N,O-sulfated K5 derivatives affect both HSPG/FGF2/FGFR and FGF2/αvβ3 interactions and are endowed with FGF2 antagonist and antiangiogenic activity. These compounds may provide the basis for the design of novel LMW heparin-like angiostatic compounds.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 2005

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Fibroblast Growth Factors
Low Molecular Weight Heparin
Fibroblast Growth Factor 2
Escherichia coli
Heparan Sulfate Proteoglycans
Heparin
Endothelial Cells
Integrins
capsular polysaccharide K5
Chorioallantoic Membrane
Surface Plasmon Resonance
Receptor Protein-Tyrosine Kinases
Chick Embryo
Cell Proliferation
Clinical Trials

Keywords

  • Angiogenesis
  • Endothelium
  • FGF
  • Heparin
  • Integrin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Antiangiogenic activity of semisynthetic biotechnological heparins : Low-molecular-weight-sulfated Escherichia coli K5 polysaccharide derivatives as fibroblast growth factor antagonists. / Presta, Marco; Oreste, Pasqua; Zoppetti, Giorgio; Belleri, Mirella; Tanghetti, Elena; Leali, Daria; Urbinati, Chiara; Bugatti, Antonella; Ronca, Roberto; Nicoli, Stefania; Moroni, Emanuela; Stabile, Helena; Camozzi, Maura; Hernandez, German Andrés; Mitola, Stefania; Dell'Era, Patrizia; Rusnati, Marco; Ribatti, Domenico.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 25, No. 1, 01.2005, p. 71-76.

Research output: Contribution to journalArticle

Presta, M, Oreste, P, Zoppetti, G, Belleri, M, Tanghetti, E, Leali, D, Urbinati, C, Bugatti, A, Ronca, R, Nicoli, S, Moroni, E, Stabile, H, Camozzi, M, Hernandez, GA, Mitola, S, Dell'Era, P, Rusnati, M & Ribatti, D 2005, 'Antiangiogenic activity of semisynthetic biotechnological heparins: Low-molecular-weight-sulfated Escherichia coli K5 polysaccharide derivatives as fibroblast growth factor antagonists', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 25, no. 1, pp. 71-76. https://doi.org/10.1161/01.ATV.0000148863.24445.b4
Presta, Marco ; Oreste, Pasqua ; Zoppetti, Giorgio ; Belleri, Mirella ; Tanghetti, Elena ; Leali, Daria ; Urbinati, Chiara ; Bugatti, Antonella ; Ronca, Roberto ; Nicoli, Stefania ; Moroni, Emanuela ; Stabile, Helena ; Camozzi, Maura ; Hernandez, German Andrés ; Mitola, Stefania ; Dell'Era, Patrizia ; Rusnati, Marco ; Ribatti, Domenico. / Antiangiogenic activity of semisynthetic biotechnological heparins : Low-molecular-weight-sulfated Escherichia coli K5 polysaccharide derivatives as fibroblast growth factor antagonists. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2005 ; Vol. 25, No. 1. pp. 71-76.
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T2 - Low-molecular-weight-sulfated Escherichia coli K5 polysaccharide derivatives as fibroblast growth factor antagonists

AU - Presta, Marco

AU - Oreste, Pasqua

AU - Zoppetti, Giorgio

AU - Belleri, Mirella

AU - Tanghetti, Elena

AU - Leali, Daria

AU - Urbinati, Chiara

AU - Bugatti, Antonella

AU - Ronca, Roberto

AU - Nicoli, Stefania

AU - Moroni, Emanuela

AU - Stabile, Helena

AU - Camozzi, Maura

AU - Hernandez, German Andrés

AU - Mitola, Stefania

AU - Dell'Era, Patrizia

AU - Rusnati, Marco

AU - Ribatti, Domenico

PY - 2005/1

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N2 - Objective - Low-molecular-weight heparin (LMWH) exerts antitumor activity in clinical trials. The K5 polysaccharide from Escherichia coli has the same structure as the heparin precursor. Chemical and enzymatic modifications of K5 polysaccharide lead to the production of biotechnological heparin-like compounds. We investigated the fibroblast growth factor-2 (FGF2) antagonist and antiangiogenic activity of a series of LMW N,O-sulfated K5 derivatives. Methods and Results - Surface plasmon resonance analysis showed that LMW-K5 derivatives bind FGF2, thus inhibiting its interaction with heparin immobilized to a BIAcore sensor chip. Interaction of FGF2 with tyrosine-kinase receptors (FGFRs), heparan sulfate proteoglycans (HSPGs), and αvβ3 integrin is required for biological response in endothelial cells. Similar to LMWH, LMW-K5 derivatives abrogate the formation of HSPG/FGF2/FGFR ternary complexes by preventing FGF2-mediated attachment of FGFR1-overexpressing cells to HSPG-bearing cells and inhibit FGF2-mediated endothelial cell proliferation. However, LMW-K5 derivatives, but not LMWH, also inhibit FGF2/α vβ3 integrin interaction and consequent FGF2-mediated endothelial cell sprouting in vitro and angiogenesis in vivo in the chick embryo chorioallantoic membrane. Conclusions - LMW N,O-sulfated K5 derivatives affect both HSPG/FGF2/FGFR and FGF2/αvβ3 interactions and are endowed with FGF2 antagonist and antiangiogenic activity. These compounds may provide the basis for the design of novel LMW heparin-like angiostatic compounds.

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KW - Integrin

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