Antiapoptotic and antigenotoxic effects of N-acetylcysteine in human cells of endothelial origin

M. G. Aluigi, S. De Flora, F. D'Agostini, A. Albini, G. Fassina

Research output: Contribution to journalArticle

Abstract

N-Acetylcysteine (NAC) is a drug bearing multiple preventive properties that can inhibit genotoxicity and carcinogenicity. NAC also inhibits invasion and metastasis of malignant cells, as well as tumor take. We recently demonstrated the effects of NAC on Kaposi's sarcoma cells supernatant-induced invasion in vitro and angiogenesis in vivo. Many anticancer agents act through cytotoxicity of rapidly proliferating cells and several antineoplastic drugs induce apoptosis of cancer cells. Since endothelial cells are the target for the inhibition of angiogenesis, we wanted to verify that NAC, while inhibiting tumor vascularization and endothelial cell invasion would not induce endothelial cell apoptosis. We tested the ability of NAC to modulate apoptosis and cytogenetic damage in vitro and to promote differentiation on a reconstituted basement membrane (matrigel) in two endothelial cell lines (EAhy926 and HUVE). Treatment with NAC protected endothelial cells from TGF-β-induced apoptosis and paraquat-induced cytogenetic damage. Therefore, NAC acts as an antiangiogenic agent and, at the same time, appears to prevent apoptosis and oxygen-related genotoxicity in endothelial cells.

Original languageEnglish
Pages (from-to)3183-3187
Number of pages5
JournalAnticancer Research
Volume20
Issue number5
Publication statusPublished - 2000

Keywords

  • Apoptosis
  • Endothelial cells
  • Genotoxicity
  • N-acetylcysteine
  • Paraquat
  • TGF-β

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Aluigi, M. G., De Flora, S., D'Agostini, F., Albini, A., & Fassina, G. (2000). Antiapoptotic and antigenotoxic effects of N-acetylcysteine in human cells of endothelial origin. Anticancer Research, 20(5), 3183-3187.