TY - JOUR
T1 - Antibacterial and anti-biofilm effects of cathelicidin peptides against pathogens isolated from cystic fibrosis patients
AU - Pompilio, A.
AU - Scocchi, M.
AU - Pomponio, S.
AU - Guida, F.
AU - Di Primio, A.
AU - Fiscarelli, E.
AU - Gennaro, R.
AU - Di Bonaventura, G.
PY - 2011/9
Y1 - 2011/9
N2 - Six different cathelicidin-derived peptides were compared to tobramycin for antibacterial and anti-biofilm effects against S. aureus, P. aeruginosa, and S. maltophilia strains isolated from cystic fibrosis patients. Overall, SMAP-29, BMAP-28, and BMAP-27 showed relevant antibacterial activity (MIC 50 4-8 μg/ml), and in some cases higher than tobramycin. In contrast, indolicidin, LL-37, and Bac7(1-35) showed no significant antimicrobial activity (MIC 50 > 32 μg/ml). Killing kinetics experiments showed that in contrast to tobramycin the active cathelicidin peptides exert a rapid bactericidal activity regardless of the species tested. All three peptides significantly reduced biofilm formation by S. maltophilia and P. aeruginosa strains at 1/2× MIC, although at a lower extent than tobramycin. In addition, BMAP-28, as well as tobramycin, was also active against S. aureus biofilm formation. Preformed biofilms were significantly affected by bactericidal SMAP-29, BMAP-27 and BMAP-28 concentrations, although at a lesser extent than tobramycin. Overall, our results indicate the potential of some cathelicidin-derived peptides for the development of novel therapeutic agents for cystic fibrosis lung disease.
AB - Six different cathelicidin-derived peptides were compared to tobramycin for antibacterial and anti-biofilm effects against S. aureus, P. aeruginosa, and S. maltophilia strains isolated from cystic fibrosis patients. Overall, SMAP-29, BMAP-28, and BMAP-27 showed relevant antibacterial activity (MIC 50 4-8 μg/ml), and in some cases higher than tobramycin. In contrast, indolicidin, LL-37, and Bac7(1-35) showed no significant antimicrobial activity (MIC 50 > 32 μg/ml). Killing kinetics experiments showed that in contrast to tobramycin the active cathelicidin peptides exert a rapid bactericidal activity regardless of the species tested. All three peptides significantly reduced biofilm formation by S. maltophilia and P. aeruginosa strains at 1/2× MIC, although at a lower extent than tobramycin. In addition, BMAP-28, as well as tobramycin, was also active against S. aureus biofilm formation. Preformed biofilms were significantly affected by bactericidal SMAP-29, BMAP-27 and BMAP-28 concentrations, although at a lesser extent than tobramycin. Overall, our results indicate the potential of some cathelicidin-derived peptides for the development of novel therapeutic agents for cystic fibrosis lung disease.
KW - Antibiotic
KW - Antimicrobial peptide
KW - Cathelicidin
KW - Cystic fibrosis
KW - Multidrug-resistance
UR - http://www.scopus.com/inward/record.url?scp=80052783463&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052783463&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2011.08.002
DO - 10.1016/j.peptides.2011.08.002
M3 - Article
C2 - 21849157
AN - SCOPUS:80052783463
VL - 32
SP - 1807
EP - 1814
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 9
ER -