Antibacterial efficacy of conventional and single-use Ni-Ti endodontic instruments: An in vitro microbiological evaluation

Alberto Dagna, Carla Renata Arciola, Livia Visai, Laura Selan, Marco Colombo, Stefano Bianchi, Claudio Poggio

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: The purpose of this in vitro study was to investigate the capability of different Ni-Ti instruments in disinfecting root canals previously infected with E. faecalis. Methods: 60 single-rooted, freshly extracted teeth were selected, sterilized and inoculated with cells of E. faecalis strain. All root canals were shaped with different Ni-Ti instruments (MTwo, Revo-S, Reciproc, and OneShape) under irrigation with NaOCl and 17% EDTA solutions by the same trained operator. The number of Colony Forming Units (CFU)/ml of E. faecalis was evaluated for each group. Numeric data were submitted to statistical analysis. Results: MTwo, REVO-S, Reciproc and OneShape reported significantly lower CFU/ml values and showed no significant differences among them (p>0.05). All groups showed significantly higher CFU/ml values than not treated samples (negative control). Conclusions: All the Ni-Ti instrumentation efficiently reduced E. faecalis counts and no significant differences were found among them. Single-use files can offer antibacterial results comparable with traditional instruments.

Original languageEnglish
Pages (from-to)826-831
Number of pages6
JournalInternational Journal of Artificial Organs
Volume35
Issue number10
DOIs
Publication statusPublished - 2012

Fingerprint

Endodontics
Stem Cells
Dental Pulp Cavity
Canals
Ethylenediaminetetraacetic acid
Irrigation
Edetic Acid
Statistical methods
Tooth
In Vitro Techniques
titanium nickelide

Keywords

  • Disinfection
  • Endodontics
  • Enterecoccus faecalis
  • Ni-Ti instruments

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

Antibacterial efficacy of conventional and single-use Ni-Ti endodontic instruments : An in vitro microbiological evaluation. / Dagna, Alberto; Arciola, Carla Renata; Visai, Livia; Selan, Laura; Colombo, Marco; Bianchi, Stefano; Poggio, Claudio.

In: International Journal of Artificial Organs, Vol. 35, No. 10, 2012, p. 826-831.

Research output: Contribution to journalArticle

@article{5ae4ccec1d52457f87bfe0fd5616232e,
title = "Antibacterial efficacy of conventional and single-use Ni-Ti endodontic instruments: An in vitro microbiological evaluation",
abstract = "Purpose: The purpose of this in vitro study was to investigate the capability of different Ni-Ti instruments in disinfecting root canals previously infected with E. faecalis. Methods: 60 single-rooted, freshly extracted teeth were selected, sterilized and inoculated with cells of E. faecalis strain. All root canals were shaped with different Ni-Ti instruments (MTwo, Revo-S, Reciproc, and OneShape) under irrigation with NaOCl and 17{\%} EDTA solutions by the same trained operator. The number of Colony Forming Units (CFU)/ml of E. faecalis was evaluated for each group. Numeric data were submitted to statistical analysis. Results: MTwo, REVO-S, Reciproc and OneShape reported significantly lower CFU/ml values and showed no significant differences among them (p>0.05). All groups showed significantly higher CFU/ml values than not treated samples (negative control). Conclusions: All the Ni-Ti instrumentation efficiently reduced E. faecalis counts and no significant differences were found among them. Single-use files can offer antibacterial results comparable with traditional instruments.",
keywords = "Disinfection, Endodontics, Enterecoccus faecalis, Ni-Ti instruments",
author = "Alberto Dagna and Arciola, {Carla Renata} and Livia Visai and Laura Selan and Marco Colombo and Stefano Bianchi and Claudio Poggio",
year = "2012",
doi = "10.5301/ijao.5000160",
language = "English",
volume = "35",
pages = "826--831",
journal = "International Journal of Artificial Organs",
issn = "0391-3988",
publisher = "Wichtig Publishing",
number = "10",

}

TY - JOUR

T1 - Antibacterial efficacy of conventional and single-use Ni-Ti endodontic instruments

T2 - An in vitro microbiological evaluation

AU - Dagna, Alberto

AU - Arciola, Carla Renata

AU - Visai, Livia

AU - Selan, Laura

AU - Colombo, Marco

AU - Bianchi, Stefano

AU - Poggio, Claudio

PY - 2012

Y1 - 2012

N2 - Purpose: The purpose of this in vitro study was to investigate the capability of different Ni-Ti instruments in disinfecting root canals previously infected with E. faecalis. Methods: 60 single-rooted, freshly extracted teeth were selected, sterilized and inoculated with cells of E. faecalis strain. All root canals were shaped with different Ni-Ti instruments (MTwo, Revo-S, Reciproc, and OneShape) under irrigation with NaOCl and 17% EDTA solutions by the same trained operator. The number of Colony Forming Units (CFU)/ml of E. faecalis was evaluated for each group. Numeric data were submitted to statistical analysis. Results: MTwo, REVO-S, Reciproc and OneShape reported significantly lower CFU/ml values and showed no significant differences among them (p>0.05). All groups showed significantly higher CFU/ml values than not treated samples (negative control). Conclusions: All the Ni-Ti instrumentation efficiently reduced E. faecalis counts and no significant differences were found among them. Single-use files can offer antibacterial results comparable with traditional instruments.

AB - Purpose: The purpose of this in vitro study was to investigate the capability of different Ni-Ti instruments in disinfecting root canals previously infected with E. faecalis. Methods: 60 single-rooted, freshly extracted teeth were selected, sterilized and inoculated with cells of E. faecalis strain. All root canals were shaped with different Ni-Ti instruments (MTwo, Revo-S, Reciproc, and OneShape) under irrigation with NaOCl and 17% EDTA solutions by the same trained operator. The number of Colony Forming Units (CFU)/ml of E. faecalis was evaluated for each group. Numeric data were submitted to statistical analysis. Results: MTwo, REVO-S, Reciproc and OneShape reported significantly lower CFU/ml values and showed no significant differences among them (p>0.05). All groups showed significantly higher CFU/ml values than not treated samples (negative control). Conclusions: All the Ni-Ti instrumentation efficiently reduced E. faecalis counts and no significant differences were found among them. Single-use files can offer antibacterial results comparable with traditional instruments.

KW - Disinfection

KW - Endodontics

KW - Enterecoccus faecalis

KW - Ni-Ti instruments

UR - http://www.scopus.com/inward/record.url?scp=84870511094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870511094&partnerID=8YFLogxK

U2 - 10.5301/ijao.5000160

DO - 10.5301/ijao.5000160

M3 - Article

C2 - 23080217

AN - SCOPUS:84870511094

VL - 35

SP - 826

EP - 831

JO - International Journal of Artificial Organs

JF - International Journal of Artificial Organs

SN - 0391-3988

IS - 10

ER -