Antibacterial Properties of a Novel Zirconium Phosphate-Glycinediphosphonate Loaded with Either Zinc or Silver

Davide Campoccia, Stefano Ravaioli, Riccardo Vivani, Anna Donnadio, Eleonora Vischini, Alessandro Russo, Livia Visai, Carla Renata Arciola, Lucio Montanaro, Morena Nocchetti

Research output: Contribution to journalArticlepeer-review


A novel compound consisting of a zirconium phosphate-glycinediphosphonate (ZPGly) has recently been introduced. This 2D-structured material forming nanosheets was exfoliated under appropriate conditions, producing colloidal aqueous dispersions (ZPGly-e) which were then loaded with zinc (Zn/ZPGly) or silver ions. Silver ions were subsequently reduced to produce metallic silver nanoparticles on exfoliated ZPGly nanosheets (Ag@ZPGly). In the search for new anti-infective materials, the present study investigated the properties of colloidal dispersions of ZPGly-e, Zn/ZPGly, and Ag@ZPGly. Ag@ZPGly was found to be a bactericidal material and was assayed to define its minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) on the five most prevalent pathogens of orthopaedic implant infections, namely: Staphylococcus aureus ATCC25923, Staphylococcus epidermidis RP62A, Enterococcus faecalis ATCC29212, Escherichia coli ATCC51739, and Pseudomonas aeruginosa ATCC27853. MIC and MBC were in the range of 125-250 μg/mL and 125-1000 μg/mL, respectively, with E. coli being the most sensitive species. Even colloidal suspensions of exfoliated ZPGly nanosheets and Zn/ZPGly exhibited some intrinsic antibacterial properties, but only at greater concentrations. Unexpectedly, Zn/ZPGly was less active than ZPGly-e.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
Issue number19
Publication statusPublished - Sep 28 2019


  • E. coli
  • E. faecalis
  • P. aeruginosa
  • S. aureus
  • S. epidermidis
  • antibacterial activity
  • implant infections
  • silver nanoparticles
  • zirconium


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