Antibiofilm activity of a monolayer of silver nanoparticles anchored to an amino-silanized glass surface

Angelo Taglietti, Carla Renata Arciola, Agnese D'Agostino, Giacomo Dacarro, Lucio Montanaro, Davide Campoccia, Lucia Cucca, Marco Vercellino, Alessandro Poggi, Piersandro Pallavicini, Livia Visai

Research output: Contribution to journalArticlepeer-review


Biofilm production is the crucial pathogenic mechanism of the implant-associated infection and a primary target for new anti-infective strategies. Silver nanoparticles (AgNPs) are attracting interest for their multifaceted potential biomedical applications. As endowed with highest surface/mass ratio and potent antibacterial activity, they can profitably be applied as monolayers at biomaterial surfaces. Desirably, in order to minimize the risks of toxic effects from freely circulating detached nanoparticles, AgNPs should firmly be anchored to the modified biomaterial surfaces. Here we focus on a newly designed glass surface modified with AgNPs and on its antibiofilm properties. Link of a self-assembled monolayer of AgNPs to glass was obtained through preliminary amino-silanization of the glass followed by immersion in an AgNPs colloidal suspension. Static contact angle measure, AFM, TEM, UV-Vis spectroscopy, ICP atomic emission spectroscopy were used for characterization. Antibiofilm activity against the biofilm-producer Staphylococcus epidermidis RP62A was assayed by both CFU method and CLSM. Performances of AgNPs-glasses were: i) excellent stability in aqueous medium; ii) prolonged release and high local concentration of Ag+ without any detaching of AgNPs; iii) strong antibiofilm activity against S. epidermidis RP62A. This AgNPs surface-modification can be applied to a large variety of biomaterials by simply depositing glass-like SiO2 films on their surfaces.

Original languageEnglish
Pages (from-to)1779-1788
Number of pages10
Issue number6
Publication statusPublished - Feb 2014


  • Anti-infective biomaterials
  • Biofilm
  • Silver nanoparticles
  • Staphylococcus epidermidis

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics


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