TY - JOUR
T1 - Antibiofilm activity of a monolayer of silver nanoparticles anchored to an amino-silanized glass surface
AU - Taglietti, Angelo
AU - Arciola, Carla Renata
AU - D'Agostino, Agnese
AU - Dacarro, Giacomo
AU - Montanaro, Lucio
AU - Campoccia, Davide
AU - Cucca, Lucia
AU - Vercellino, Marco
AU - Poggi, Alessandro
AU - Pallavicini, Piersandro
AU - Visai, Livia
PY - 2014/2
Y1 - 2014/2
N2 - Biofilm production is the crucial pathogenic mechanism of the implant-associated infection and a primary target for new anti-infective strategies. Silver nanoparticles (AgNPs) are attracting interest for their multifaceted potential biomedical applications. As endowed with highest surface/mass ratio and potent antibacterial activity, they can profitably be applied as monolayers at biomaterial surfaces. Desirably, in order to minimize the risks of toxic effects from freely circulating detached nanoparticles, AgNPs should firmly be anchored to the modified biomaterial surfaces. Here we focus on a newly designed glass surface modified with AgNPs and on its antibiofilm properties. Link of a self-assembled monolayer of AgNPs to glass was obtained through preliminary amino-silanization of the glass followed by immersion in an AgNPs colloidal suspension. Static contact angle measure, AFM, TEM, UV-Vis spectroscopy, ICP atomic emission spectroscopy were used for characterization. Antibiofilm activity against the biofilm-producer Staphylococcus epidermidis RP62A was assayed by both CFU method and CLSM. Performances of AgNPs-glasses were: i) excellent stability in aqueous medium; ii) prolonged release and high local concentration of Ag+ without any detaching of AgNPs; iii) strong antibiofilm activity against S. epidermidis RP62A. This AgNPs surface-modification can be applied to a large variety of biomaterials by simply depositing glass-like SiO2 films on their surfaces.
AB - Biofilm production is the crucial pathogenic mechanism of the implant-associated infection and a primary target for new anti-infective strategies. Silver nanoparticles (AgNPs) are attracting interest for their multifaceted potential biomedical applications. As endowed with highest surface/mass ratio and potent antibacterial activity, they can profitably be applied as monolayers at biomaterial surfaces. Desirably, in order to minimize the risks of toxic effects from freely circulating detached nanoparticles, AgNPs should firmly be anchored to the modified biomaterial surfaces. Here we focus on a newly designed glass surface modified with AgNPs and on its antibiofilm properties. Link of a self-assembled monolayer of AgNPs to glass was obtained through preliminary amino-silanization of the glass followed by immersion in an AgNPs colloidal suspension. Static contact angle measure, AFM, TEM, UV-Vis spectroscopy, ICP atomic emission spectroscopy were used for characterization. Antibiofilm activity against the biofilm-producer Staphylococcus epidermidis RP62A was assayed by both CFU method and CLSM. Performances of AgNPs-glasses were: i) excellent stability in aqueous medium; ii) prolonged release and high local concentration of Ag+ without any detaching of AgNPs; iii) strong antibiofilm activity against S. epidermidis RP62A. This AgNPs surface-modification can be applied to a large variety of biomaterials by simply depositing glass-like SiO2 films on their surfaces.
KW - Anti-infective biomaterials
KW - APTES
KW - Biofilm
KW - Silver nanoparticles
KW - Staphylococcus epidermidis
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U2 - 10.1016/j.biomaterials.2013.11.047
DO - 10.1016/j.biomaterials.2013.11.047
M3 - Article
C2 - 24315574
AN - SCOPUS:84890245972
VL - 35
SP - 1779
EP - 1788
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 6
ER -