Antibiofilm activity of sandblasted and laser-modified titanium against microorganisms isolated from peri-implantitis lesions

Lorenzo Drago, Monica Bortolin, Elena De Vecchi, Serse Agrappi, Roberto L. Weinstein, Roberto Mattina, Luca Francetti

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Infections due to biofilm-producing microorganisms are one of the main causes for the failure of dental implants. Increasing efforts have been made in order to develop new strategies to prevent biofilm formation. In this study, the biofilm development on a newly designed laser-modified titanium implant surface was evaluated and compared to that on conventional sandblasted titanium used in implant dentistry. The amount of biofilm produced by Staphylococcus aureus, Pseudomonas aeruginosa and Porphyromonas gingivalis isolated from peri-implantitis was assessed by a semi-quantitative spectrophotometric method and by confocal laser scanning microscopy. Results showed a lower biofilm production on laser-modified surface compared to the sandblasted one. In particular, a significantly lower total volume of the biomass was observed on laser-modified surface, while no significant changes in live/dead bacteria percentages were noticed between materials. Modifying the topography of the conventional implant surface with laser ablation could represent a promising approach for inhibiting biofilm formation.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Chemotherapy
DOIs
Publication statusAccepted/In press - May 29 2016

Fingerprint

Peri-Implantitis
Biofilms
Titanium
Lasers
Porphyromonas gingivalis
Dental Implants
Laser Therapy
Dentistry
Confocal Microscopy
Biomass
Pseudomonas aeruginosa
Staphylococcus aureus
Bacteria
Infection

Keywords

  • Biofilm
  • Confocal laser scanning microscopy
  • Dental implants
  • Dental materials
  • Titanium oxide

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Infectious Diseases
  • Oncology
  • Pharmacology

Cite this

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title = "Antibiofilm activity of sandblasted and laser-modified titanium against microorganisms isolated from peri-implantitis lesions",
abstract = "Infections due to biofilm-producing microorganisms are one of the main causes for the failure of dental implants. Increasing efforts have been made in order to develop new strategies to prevent biofilm formation. In this study, the biofilm development on a newly designed laser-modified titanium implant surface was evaluated and compared to that on conventional sandblasted titanium used in implant dentistry. The amount of biofilm produced by Staphylococcus aureus, Pseudomonas aeruginosa and Porphyromonas gingivalis isolated from peri-implantitis was assessed by a semi-quantitative spectrophotometric method and by confocal laser scanning microscopy. Results showed a lower biofilm production on laser-modified surface compared to the sandblasted one. In particular, a significantly lower total volume of the biomass was observed on laser-modified surface, while no significant changes in live/dead bacteria percentages were noticed between materials. Modifying the topography of the conventional implant surface with laser ablation could represent a promising approach for inhibiting biofilm formation.",
keywords = "Biofilm, Confocal laser scanning microscopy, Dental implants, Dental materials, Titanium oxide",
author = "Lorenzo Drago and Monica Bortolin and {De Vecchi}, Elena and Serse Agrappi and Weinstein, {Roberto L.} and Roberto Mattina and Luca Francetti",
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AU - Drago, Lorenzo

AU - Bortolin, Monica

AU - De Vecchi, Elena

AU - Agrappi, Serse

AU - Weinstein, Roberto L.

AU - Mattina, Roberto

AU - Francetti, Luca

PY - 2016/5/29

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N2 - Infections due to biofilm-producing microorganisms are one of the main causes for the failure of dental implants. Increasing efforts have been made in order to develop new strategies to prevent biofilm formation. In this study, the biofilm development on a newly designed laser-modified titanium implant surface was evaluated and compared to that on conventional sandblasted titanium used in implant dentistry. The amount of biofilm produced by Staphylococcus aureus, Pseudomonas aeruginosa and Porphyromonas gingivalis isolated from peri-implantitis was assessed by a semi-quantitative spectrophotometric method and by confocal laser scanning microscopy. Results showed a lower biofilm production on laser-modified surface compared to the sandblasted one. In particular, a significantly lower total volume of the biomass was observed on laser-modified surface, while no significant changes in live/dead bacteria percentages were noticed between materials. Modifying the topography of the conventional implant surface with laser ablation could represent a promising approach for inhibiting biofilm formation.

AB - Infections due to biofilm-producing microorganisms are one of the main causes for the failure of dental implants. Increasing efforts have been made in order to develop new strategies to prevent biofilm formation. In this study, the biofilm development on a newly designed laser-modified titanium implant surface was evaluated and compared to that on conventional sandblasted titanium used in implant dentistry. The amount of biofilm produced by Staphylococcus aureus, Pseudomonas aeruginosa and Porphyromonas gingivalis isolated from peri-implantitis was assessed by a semi-quantitative spectrophotometric method and by confocal laser scanning microscopy. Results showed a lower biofilm production on laser-modified surface compared to the sandblasted one. In particular, a significantly lower total volume of the biomass was observed on laser-modified surface, while no significant changes in live/dead bacteria percentages were noticed between materials. Modifying the topography of the conventional implant surface with laser ablation could represent a promising approach for inhibiting biofilm formation.

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