Antibiotic-impregnated fibrin glue in ocular surgery: In vitro antibacterial activity

Research output: Contribution to journalArticle

Abstract

A strategy to reduce postoperative ocular infections might consist in maintaining a high antibiotic concentration in the eye by using a topical slow-release drug formulation. Fibrin glue is widely used in ocular surgery. Vancomycin, teicoplanin, cephalothin and gentamicin were added to a 500 IU/ml thrombin solution, their concentrations in 1 g fibrin glue being 500 μg for vancomycin and 400 μg for the other drugs. The in vitro antibacterial activity of antibiotic-impregnated fibrin glue was evaluated against a recent clinical isolate of Staphylococcus epidermidis. Moreover the antibiotic elution from fibrin discs in saline was assayed for up to 96 h. Fibrin glue properties were maintained when thrombin was reconstituted with a CaCl 2 solution additioned with the antibiotics tested. All antibiotic fibrin mixtures exhibited good antistaphylococcal activity immediately after the preparation. A prolonged inhibition (4 days) of S. epidermidis growth was observed with teicoplanin discs. Gentamicin and teicoplanin levels were higher than those of the other antibiotics. The results suggest that the fibrin glycopeptide and/or gentamicin mixtures can be used, both prophylactically and therapeutically, in ocular surgery.

Original languageEnglish
Pages (from-to)12-15
Number of pages4
JournalOphthalmologica
Volume213
Issue number1
DOIs
Publication statusPublished - 1999

Keywords

  • Antibacterial activity
  • Antibiotic-fibrin mixtures
  • Fibrin glue
  • Ocular surgery
  • Postoperative ocular infections

ASJC Scopus subject areas

  • Ophthalmology

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