Antibiotic-induced dysbiosis of the microbiota impairs gut neuromuscular function in juvenile mice

Valentina Caputi, Ilaria Marsilio, Viviana Filpa, Silvia Cerantola, Genny Orso, Michela Bistoletti, Nicola Paccagnella, Sara De Martin, Monica Montopoli, Stefano Dall'Acqua, Francesca Crema, Iole Maria Di Gangi, Francesca Galuppini, Isabella Lante, Sara Bogialli, Massimo Rugge, Patrizia Debetto, Cristina Giaroni, Maria Cecilia Giron

Research output: Contribution to journalArticle

Abstract

Background and Purpose: Gut microbiota is essential for the development of the gastrointestinal system, including the enteric nervous system (ENS). Perturbations of gut microbiota in early life have the potential to alter neurodevelopment leading to functional bowel disorders later in life. We examined the hypothesis that gut dysbiosis impairs the structural and functional integrity of the ENS, leading to gut dysmotility in juvenile mice. Experimental Approach: To induce gut dysbiosis, broad-spectrum antibiotics were administered by gavage to juvenile (3weeks old) male C57Bl/6 mice for 14 days. Bile acid composition in the intestinal lumen was analysed by liquid chromatography-mass spectrometry. Changes in intestinal motility were evaluated by stool frequency, transit of a fluorescent-labelled marker and isometric muscle responses of ileal full-thickness preparations to receptor and non-receptor-mediated stimuli. Alterations in ENS integrity were assessed by immunohistochemistry and Western blot analysis. Key Results: Antibiotic treatment altered gastrointestinal transit, luminal bile acid metabolism and bowel architecture. Gut dysbiosis resulted in distorted glial network, loss of myenteric plexus neurons, altered cholinergic, tachykininergic and nitrergic neurotransmission associated with reduced number of nNOS neurons and different ileal distribution of the toll-like receptor TLR2. Functional defects were partly reversed by activation of TLR2 signalling. Conclusions and Implications: Gut dysbiosis caused complex morpho-functional neuromuscular rearrangements, characterized by structural defects of the ENS and increased tachykininergic neurotransmission. Altogether, our findings support the beneficial role of enteric microbiota for ENS homeostasis instrumental in ensuring proper gut neuromuscular function during critical stages of development.

Original languageEnglish
Pages (from-to)3623-3639
Number of pages17
JournalBritish Journal of Pharmacology
Volume174
Issue number20
DOIs
Publication statusPublished - Jan 1 2017

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Dysbiosis
Enteric Nervous System
Anti-Bacterial Agents
Bile Acids and Salts
Synaptic Transmission
Nitrergic Neurons
Gastrointestinal Transit
Myenteric Plexus
Cholinergic Neurons
Gastrointestinal Motility
Toll-Like Receptors
Microbiota
Neuroglia
Liquid Chromatography
Mass Spectrometry
Homeostasis
Western Blotting
Immunohistochemistry
Gastrointestinal Microbiome
Neurons

ASJC Scopus subject areas

  • Pharmacology

Cite this

Caputi, V., Marsilio, I., Filpa, V., Cerantola, S., Orso, G., Bistoletti, M., ... Giron, M. C. (2017). Antibiotic-induced dysbiosis of the microbiota impairs gut neuromuscular function in juvenile mice. British Journal of Pharmacology, 174(20), 3623-3639. https://doi.org/10.1111/bph.13965

Antibiotic-induced dysbiosis of the microbiota impairs gut neuromuscular function in juvenile mice. / Caputi, Valentina; Marsilio, Ilaria; Filpa, Viviana; Cerantola, Silvia; Orso, Genny; Bistoletti, Michela; Paccagnella, Nicola; De Martin, Sara; Montopoli, Monica; Dall'Acqua, Stefano; Crema, Francesca; Di Gangi, Iole Maria; Galuppini, Francesca; Lante, Isabella; Bogialli, Sara; Rugge, Massimo; Debetto, Patrizia; Giaroni, Cristina; Giron, Maria Cecilia.

In: British Journal of Pharmacology, Vol. 174, No. 20, 01.01.2017, p. 3623-3639.

Research output: Contribution to journalArticle

Caputi, V, Marsilio, I, Filpa, V, Cerantola, S, Orso, G, Bistoletti, M, Paccagnella, N, De Martin, S, Montopoli, M, Dall'Acqua, S, Crema, F, Di Gangi, IM, Galuppini, F, Lante, I, Bogialli, S, Rugge, M, Debetto, P, Giaroni, C & Giron, MC 2017, 'Antibiotic-induced dysbiosis of the microbiota impairs gut neuromuscular function in juvenile mice', British Journal of Pharmacology, vol. 174, no. 20, pp. 3623-3639. https://doi.org/10.1111/bph.13965
Caputi, Valentina ; Marsilio, Ilaria ; Filpa, Viviana ; Cerantola, Silvia ; Orso, Genny ; Bistoletti, Michela ; Paccagnella, Nicola ; De Martin, Sara ; Montopoli, Monica ; Dall'Acqua, Stefano ; Crema, Francesca ; Di Gangi, Iole Maria ; Galuppini, Francesca ; Lante, Isabella ; Bogialli, Sara ; Rugge, Massimo ; Debetto, Patrizia ; Giaroni, Cristina ; Giron, Maria Cecilia. / Antibiotic-induced dysbiosis of the microbiota impairs gut neuromuscular function in juvenile mice. In: British Journal of Pharmacology. 2017 ; Vol. 174, No. 20. pp. 3623-3639.
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author = "Valentina Caputi and Ilaria Marsilio and Viviana Filpa and Silvia Cerantola and Genny Orso and Michela Bistoletti and Nicola Paccagnella and {De Martin}, Sara and Monica Montopoli and Stefano Dall'Acqua and Francesca Crema and {Di Gangi}, {Iole Maria} and Francesca Galuppini and Isabella Lante and Sara Bogialli and Massimo Rugge and Patrizia Debetto and Cristina Giaroni and Giron, {Maria Cecilia}",
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AU - Caputi, Valentina

AU - Marsilio, Ilaria

AU - Filpa, Viviana

AU - Cerantola, Silvia

AU - Orso, Genny

AU - Bistoletti, Michela

AU - Paccagnella, Nicola

AU - De Martin, Sara

AU - Montopoli, Monica

AU - Dall'Acqua, Stefano

AU - Crema, Francesca

AU - Di Gangi, Iole Maria

AU - Galuppini, Francesca

AU - Lante, Isabella

AU - Bogialli, Sara

AU - Rugge, Massimo

AU - Debetto, Patrizia

AU - Giaroni, Cristina

AU - Giron, Maria Cecilia

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background and Purpose: Gut microbiota is essential for the development of the gastrointestinal system, including the enteric nervous system (ENS). Perturbations of gut microbiota in early life have the potential to alter neurodevelopment leading to functional bowel disorders later in life. We examined the hypothesis that gut dysbiosis impairs the structural and functional integrity of the ENS, leading to gut dysmotility in juvenile mice. Experimental Approach: To induce gut dysbiosis, broad-spectrum antibiotics were administered by gavage to juvenile (3weeks old) male C57Bl/6 mice for 14 days. Bile acid composition in the intestinal lumen was analysed by liquid chromatography-mass spectrometry. Changes in intestinal motility were evaluated by stool frequency, transit of a fluorescent-labelled marker and isometric muscle responses of ileal full-thickness preparations to receptor and non-receptor-mediated stimuli. Alterations in ENS integrity were assessed by immunohistochemistry and Western blot analysis. Key Results: Antibiotic treatment altered gastrointestinal transit, luminal bile acid metabolism and bowel architecture. Gut dysbiosis resulted in distorted glial network, loss of myenteric plexus neurons, altered cholinergic, tachykininergic and nitrergic neurotransmission associated with reduced number of nNOS neurons and different ileal distribution of the toll-like receptor TLR2. Functional defects were partly reversed by activation of TLR2 signalling. Conclusions and Implications: Gut dysbiosis caused complex morpho-functional neuromuscular rearrangements, characterized by structural defects of the ENS and increased tachykininergic neurotransmission. Altogether, our findings support the beneficial role of enteric microbiota for ENS homeostasis instrumental in ensuring proper gut neuromuscular function during critical stages of development.

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