Antibodies against sialophorin (CD43) enhance the capacity of dendritic cells to cluster and activate T lymphocytes

Emanuele Fanales-Belasio, Giovanna Zambruno, Andrea Cavani, Giampiero Girolomoni

Research output: Contribution to journalArticle

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Abstract

CD43 is a leukocyte membrane glycoprotein rich in negatively charged sialic acid residues that may act as an anti-adhesion molecule. In this study, we analyzed the expression and function of CD43 on epidermal Langerhans cells (LC) and dendritic cells derived from adherent peripheral blood monocytes treated with granulocyte-macrophage CSF and IL-4 (Mo-DC). Both freshly isolated LC (fLC) and Mo-DC expressed uniformly high amounts of membrane CD43, with levels unchanged in 48-h cultured LC or in TNF-α-treated Mo-DC. When fLC or Mo-DC were transiently preincubated with anti-CD43 mAb or neuraminidase, they formed much larger clusters with T cells as compared with cells incubated with control mAb. Moreover, a significantly increased proliferation of naive allogeneic T cells in a primary MLR assay was induced. Anti-CD43-treated Mo-DC also presented tetanus toxoid to primed T cells more efficiently. Immunofluorescence and immunogold electron microscopy showed that following Ab cross-linking and warming to 37°C, CD43 is progressively removed from the cell surface and is internalized within endosomes and vacuoles. In addition, fLC and Mo-DC incubated with anti-CD43 mAb increased expression of HLA-DR, ICAM-1, B7-1, B7-2, CD40, and CD83 after 24 h, and this was partially prevented by culturing cells in the presence of cycloheximide. We thus postulate that membrane CD43, other than serving as anti-adhesion molecule, can mediate activation signals in immature DC, leading to augmented expression of adhesion/costimulatory molecules and Ag-presenting function.

Original languageEnglish
Pages (from-to)2203-2211
Number of pages9
JournalJournal of Immunology
Volume159
Issue number5
Publication statusPublished - 1997

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CD43 Antigens
Langerhans Cells
Dendritic Cells
T-Lymphocytes
Antibodies
Tetanus Toxoid
Membranes
Endosomes
Membrane Glycoproteins
Neuraminidase
HLA-DR Antigens
N-Acetylneuraminic Acid
Intercellular Adhesion Molecule-1
Cycloheximide
Vacuoles
Granulocytes
Interleukin-4
Fluorescent Antibody Technique
Monocytes
Cultured Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Antibodies against sialophorin (CD43) enhance the capacity of dendritic cells to cluster and activate T lymphocytes. / Fanales-Belasio, Emanuele; Zambruno, Giovanna; Cavani, Andrea; Girolomoni, Giampiero.

In: Journal of Immunology, Vol. 159, No. 5, 1997, p. 2203-2211.

Research output: Contribution to journalArticle

Fanales-Belasio, Emanuele ; Zambruno, Giovanna ; Cavani, Andrea ; Girolomoni, Giampiero. / Antibodies against sialophorin (CD43) enhance the capacity of dendritic cells to cluster and activate T lymphocytes. In: Journal of Immunology. 1997 ; Vol. 159, No. 5. pp. 2203-2211.
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