Antibodies are forever: A study using 12-26-year-old expired antibodies

Maria C. Argentieri; Daniela Pilla; Alice Vanzati; Silvia Lonardi; Fabio Facchetti; Claudio Doglioni; Carlo Parravicini; Giorgio Cattoretti

doi:10.1111/his.12225

Antibodies are forever: A study using 12-26-year-old expired antibodies

Maria C. Argentieri, Daniela Pilla, Alice Vanzati, Silvia Lonardi, Fabio Facchetti, Claudio Doglioni, Carlo Parravicini, Giorgio Cattoretti

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Aims: The aim of this study was to investigate whether the shelf-life of diagnostic antibodies is longer than the expiry date on the label. Methods and results: Four independent laboratories tested a small number of diagnostic antibodies kept at +4°C for 12-26 years, and found them to work perfectly on routine histology sections. Conclusions: Diagnostic antibodies may have a workable half-life in excess of 10 years, and the emphasis on performance should shift to the preservation of antigenic targets in the tissue.

Original language	English
Pages (from-to)	869-876
Number of pages	8
Journal	Histopathology
Volume	63
Issue number	6
DOIs	https://doi.org/10.1111/his.12225
Publication status	Published - Dec 2013

Keywords

Antibody
Expiry date
Immunohistochemistry

ASJC Scopus subject areas

Histology
Pathology and Forensic Medicine

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10.1111/his.12225

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title = "Antibodies are forever: A study using 12-26-year-old expired antibodies",

abstract = "Aims: The aim of this study was to investigate whether the shelf-life of diagnostic antibodies is longer than the expiry date on the label. Methods and results: Four independent laboratories tested a small number of diagnostic antibodies kept at +4°C for 12-26 years, and found them to work perfectly on routine histology sections. Conclusions: Diagnostic antibodies may have a workable half-life in excess of 10 years, and the emphasis on performance should shift to the preservation of antigenic targets in the tissue.",

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AU - Argentieri, Maria C.

AU - Pilla, Daniela

AU - Vanzati, Alice

AU - Lonardi, Silvia

AU - Facchetti, Fabio

AU - Doglioni, Claudio

AU - Parravicini, Carlo

AU - Cattoretti, Giorgio

PY - 2013/12

Y1 - 2013/12

N2 - Aims: The aim of this study was to investigate whether the shelf-life of diagnostic antibodies is longer than the expiry date on the label. Methods and results: Four independent laboratories tested a small number of diagnostic antibodies kept at +4°C for 12-26 years, and found them to work perfectly on routine histology sections. Conclusions: Diagnostic antibodies may have a workable half-life in excess of 10 years, and the emphasis on performance should shift to the preservation of antigenic targets in the tissue.

AB - Aims: The aim of this study was to investigate whether the shelf-life of diagnostic antibodies is longer than the expiry date on the label. Methods and results: Four independent laboratories tested a small number of diagnostic antibodies kept at +4°C for 12-26 years, and found them to work perfectly on routine histology sections. Conclusions: Diagnostic antibodies may have a workable half-life in excess of 10 years, and the emphasis on performance should shift to the preservation of antigenic targets in the tissue.

KW - Antibody

KW - Expiry date

KW - Immunohistochemistry

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ER -

Antibodies are forever: A study using 12-26-year-old expired antibodies

Abstract

Keywords

ASJC Scopus subject areas

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