Antibodies binding granulocyte-macrophage colony stimulating factor produced by cord blood-derived B cell lines immortalized by Epstein-Barr virus in vitro

Roberto P. Revoltella, Leopoldo Laricchia Robbio, Anna Marina Liberati, Gigliola Reato, Robin Foa, Ada Funaro, Fabrizio Vinante, Giovanni Pizzolo

Research output: Contribution to journalArticle

Abstract

We detected natural antibodies (auto-Abs) binding human granulocyte-macrophage colony stimulating factor (GM-CSF) in umbilical cord blood (CB) (23 of 94 samples screened) and peripheral blood of women at the end of pregnancy (6 of 42 samples tested). To demonstrate that Abs detected in CB were produced by the fetus, CB mononuclear cells were infected with Epstein-Barr virus in vitro. Ten cell lines producing constitutively anti-recombinant human GM-CSF (rhGM-CSF) Abs were isolated and characterized. These cells displayed a male karyotype, an early activated B cell phenotype, coexpressed surface IgM and IgD, and secreted only IgM with prevailing λ clonal restriction. Specific cell surface binding of biotinylated rhGM-CSF and high-level anti-rhGM-CSF IgM Ab production were typical features of early cell cultures. In late cell passages the frequency of more undifferentiated B cells increased. Serum Abs of either maternal or fetal origin or Abs produced in culture did not affect the granulocyte and macrophage colony stimulating activity of rhGM-CSF from bone marrow progenitors in soft agar, suggesting that the Abs produced were non-neutralizing. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)114-127
Number of pages14
JournalCellular Immunology
Volume204
Issue number2
DOIs
Publication statusPublished - Sep 15 2000

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Granulocyte-Macrophage Colony-Stimulating Factor
Human Herpesvirus 4
Fetal Blood
B-Lymphocytes
Cell Line
Antibodies
Immunoglobulin M
Immunoglobulin D
Karyotype
Granulocytes
Human Activities
Agar
Blood Cells
Fetus
Cell Culture Techniques
Bone Marrow
Macrophages
Mothers
In Vitro Techniques
Phenotype

Keywords

  • B cell lines
  • Epstein-Barr virus
  • Human anti-GM-CSF autoantibodies
  • Umbilical cord blood

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Antibodies binding granulocyte-macrophage colony stimulating factor produced by cord blood-derived B cell lines immortalized by Epstein-Barr virus in vitro. / Revoltella, Roberto P.; Laricchia Robbio, Leopoldo; Liberati, Anna Marina; Reato, Gigliola; Foa, Robin; Funaro, Ada; Vinante, Fabrizio; Pizzolo, Giovanni.

In: Cellular Immunology, Vol. 204, No. 2, 15.09.2000, p. 114-127.

Research output: Contribution to journalArticle

Revoltella, Roberto P. ; Laricchia Robbio, Leopoldo ; Liberati, Anna Marina ; Reato, Gigliola ; Foa, Robin ; Funaro, Ada ; Vinante, Fabrizio ; Pizzolo, Giovanni. / Antibodies binding granulocyte-macrophage colony stimulating factor produced by cord blood-derived B cell lines immortalized by Epstein-Barr virus in vitro. In: Cellular Immunology. 2000 ; Vol. 204, No. 2. pp. 114-127.
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