Antibodies for denatured human H-ferritin stain only reticuloendothelial cells within the bone marrow

G. Ruggeri, P. Santambrogio, F. Bonfiglio, S. Levi, G. Bugari, R. Verardi, M. Cazzola, R. Invernizzi, L. M. Zambelli, A. Albertini, P. Arosio

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Abstract

Human H-ferritin homopolymer was denatured in sodium dodecyl sulphate and injected in mice to obtain antibodies for dissociated H-subunit. The antisera and Moabs obtained were specific for the denatured H-chain with no cross-reactivity with assembled ferritins in immunoblotting experiments. In contrast the Moabs for native recombinant H-ferritin are specific for the assembled ferritin molecules with weak cross-reactivity with the denatured H-subunits. The epitope recognized by one of the anti-denatured H-chain Moabs was mapped on the C-terminal helix of ferritin. The antibodies were used to study H-ferritin conformation in cells. In immunocytochemistry experiments the antibodies for denatured H-ferritin stained HeLa and K562 cells weakly, with a different intensity and pattern to those obtained with anti-native H-ferritin antibody. In human bone marrow smears the anti-denatured ferritin antibodies stained only reticuloendothelial cells, and did not recognize the H-ferritin rich immature erythroblasts. It is concluded that assembled and denatured H-ferritins are immunogenically distinct, and that erythroid and reticuloendothelial cells within the bone marrow contain H-ferritin in different conformations.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalBritish Journal of Haematology
Volume81
Issue number1
Publication statusPublished - 1992

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Apoferritins
Bone Marrow Cells
Coloring Agents
Antibodies
Ferritins
Bone Marrow
Erythroblasts
Erythroid Cells
K562 Cells
HeLa Cells
Immunoblotting
Sodium Dodecyl Sulfate
Immune Sera
Epitopes
Immunohistochemistry

ASJC Scopus subject areas

  • Hematology

Cite this

Antibodies for denatured human H-ferritin stain only reticuloendothelial cells within the bone marrow. / Ruggeri, G.; Santambrogio, P.; Bonfiglio, F.; Levi, S.; Bugari, G.; Verardi, R.; Cazzola, M.; Invernizzi, R.; Zambelli, L. M.; Albertini, A.; Arosio, P.

In: British Journal of Haematology, Vol. 81, No. 1, 1992, p. 118-124.

Research output: Contribution to journalArticle

Ruggeri, G, Santambrogio, P, Bonfiglio, F, Levi, S, Bugari, G, Verardi, R, Cazzola, M, Invernizzi, R, Zambelli, LM, Albertini, A & Arosio, P 1992, 'Antibodies for denatured human H-ferritin stain only reticuloendothelial cells within the bone marrow', British Journal of Haematology, vol. 81, no. 1, pp. 118-124.
Ruggeri, G. ; Santambrogio, P. ; Bonfiglio, F. ; Levi, S. ; Bugari, G. ; Verardi, R. ; Cazzola, M. ; Invernizzi, R. ; Zambelli, L. M. ; Albertini, A. ; Arosio, P. / Antibodies for denatured human H-ferritin stain only reticuloendothelial cells within the bone marrow. In: British Journal of Haematology. 1992 ; Vol. 81, No. 1. pp. 118-124.
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