Abstract
Antibodies to recombinant heterogeneous nuclear RNP core protein A1 were detected in sera from 27 of 58 patients with rheumatoid arthritis (RA) and from 7 of 31 patients with systemic lupus erythematosus, by immunoblotting and enzyme-linked immunosorbent assay. Protein A1 consists of 2 distinct domains: The N-terminal sequence is identical to a single-stranded DNA binding protein termed UP1, and the C-terminal domain shows a partial homology with keratin. All 7 A1-positive systemic lupus erythematosus sera reacted with UP1, whereas 9 of the 27 A1-positive RA sera did not. In RA, anti-A1 activity was significantly associated with antikeratin antibodies (AKA); these antibodies were present in 23 of 27 A1-positive sera and 10 of 31 A1-negative sera (P <0.01). Immunoabsorption with recombinant protein A1 resulted in a significant reduction of AKA titers in 6 of 10 RA sera tested, suggesting that AKA from RA patients may cross-react with the C-terminal portion of the heterogeneous nuclear RNP protein A1.
| Original language | English |
|---|---|
| Pages (from-to) | 180-186 |
| Number of pages | 7 |
| Journal | Arthritis and Rheumatism |
| Volume | 33 |
| Issue number | 2 |
| Publication status | Published - Feb 1990 |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Rheumatology
Cite this
Antibodies from patients with rheumatoid arthritis and systemic lupus erythematosus recognize different epitopes of a single heterogeneous nuclear RNP core protein : Possible role of cross-reacting antikeratin antibodies. / Montecucco, Carlomaurizio; Caporali, Roberto; Negri, Claudia; De Gennaro, Fabio; Cerino, Antonella; Bestagno, Marco; Cobianchi, Fabio; Astaldi-Ricotti, Giulia C B.
In: Arthritis and Rheumatism, Vol. 33, No. 2, 02.1990, p. 180-186.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibodies from patients with rheumatoid arthritis and systemic lupus erythematosus recognize different epitopes of a single heterogeneous nuclear RNP core protein
T2 - Possible role of cross-reacting antikeratin antibodies
AU - Montecucco, Carlomaurizio
AU - Caporali, Roberto
AU - Negri, Claudia
AU - De Gennaro, Fabio
AU - Cerino, Antonella
AU - Bestagno, Marco
AU - Cobianchi, Fabio
AU - Astaldi-Ricotti, Giulia C B
PY - 1990/2
Y1 - 1990/2
N2 - Antibodies to recombinant heterogeneous nuclear RNP core protein A1 were detected in sera from 27 of 58 patients with rheumatoid arthritis (RA) and from 7 of 31 patients with systemic lupus erythematosus, by immunoblotting and enzyme-linked immunosorbent assay. Protein A1 consists of 2 distinct domains: The N-terminal sequence is identical to a single-stranded DNA binding protein termed UP1, and the C-terminal domain shows a partial homology with keratin. All 7 A1-positive systemic lupus erythematosus sera reacted with UP1, whereas 9 of the 27 A1-positive RA sera did not. In RA, anti-A1 activity was significantly associated with antikeratin antibodies (AKA); these antibodies were present in 23 of 27 A1-positive sera and 10 of 31 A1-negative sera (P <0.01). Immunoabsorption with recombinant protein A1 resulted in a significant reduction of AKA titers in 6 of 10 RA sera tested, suggesting that AKA from RA patients may cross-react with the C-terminal portion of the heterogeneous nuclear RNP protein A1.
AB - Antibodies to recombinant heterogeneous nuclear RNP core protein A1 were detected in sera from 27 of 58 patients with rheumatoid arthritis (RA) and from 7 of 31 patients with systemic lupus erythematosus, by immunoblotting and enzyme-linked immunosorbent assay. Protein A1 consists of 2 distinct domains: The N-terminal sequence is identical to a single-stranded DNA binding protein termed UP1, and the C-terminal domain shows a partial homology with keratin. All 7 A1-positive systemic lupus erythematosus sera reacted with UP1, whereas 9 of the 27 A1-positive RA sera did not. In RA, anti-A1 activity was significantly associated with antikeratin antibodies (AKA); these antibodies were present in 23 of 27 A1-positive sera and 10 of 31 A1-negative sera (P <0.01). Immunoabsorption with recombinant protein A1 resulted in a significant reduction of AKA titers in 6 of 10 RA sera tested, suggesting that AKA from RA patients may cross-react with the C-terminal portion of the heterogeneous nuclear RNP protein A1.
UR - http://www.scopus.com/inward/record.url?scp=0025266662&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025266662&partnerID=8YFLogxK
M3 - Article
C2 - 1689580
AN - SCOPUS:0025266662
VL - 33
SP - 180
EP - 186
JO - Arthritis care and research : the official journal of the Arthritis Health Professions Association
JF - Arthritis care and research : the official journal of the Arthritis Health Professions Association
SN - 0893-7524
IS - 2
ER -